{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T15:07:35Z","timestamp":1779203255587,"version":"3.51.4"},"reference-count":65,"publisher":"American Society for Clinical Investigation","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,1,26]]},"abstract":"<jats:p>\n                    A role for oxidative stress in the brain has been suggested in the pathogenesis of diet-induced obesity (DIO), although the underlying neural regions and mechanisms remain incompletely defined. We tested the hypothesis that NADPH oxidase\u2013dependent oxidative stress in the paraventricular nucleus (PVN), a hypothalamic energy homeostasis center, contributes to the development of DIO. Cre\/LoxP technology was coupled with selective PVN adenoviral microinjection to ablate\n                    <jats:italic>\n                      p22\n                      <jats:sup>phox<\/jats:sup>\n                    <\/jats:italic>\n                    , the obligatory subunit for NADPH oxidase activity, in mice harboring a conditional\n                    <jats:italic>\n                      p22\n                      <jats:sup>phox<\/jats:sup>\n                    <\/jats:italic>\n                    allele. Selective deletion of\n                    <jats:italic>\n                      p22\n                      <jats:sup>phox<\/jats:sup>\n                    <\/jats:italic>\n                    in the PVN protected mice from high-fat DIO independent of changes in food intake or locomotor activity. This was accompanied by \u03b2\n                    <jats:sub>3<\/jats:sub>\n                    -adrenoceptor\u2013dependent increases in energy expenditure, elevations in brown adipose tissue thermogenesis, and browning of white adipose tissue. These data reveal a potentially novel role for brain oxidative stress in the development of DIO by modulating \u03b2\n                    <jats:sub>3<\/jats:sub>\n                    -adrenoceptor mechanisms and point to the PVN as an underlying neural site.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.87094","type":"journal-article","created":{"date-parts":[[2017,1,25]],"date-time":"2017-01-25T11:01:10Z","timestamp":1485342070000},"source":"Crossref","is-referenced-by-count":11,"title":["Deletion of p22phox-dependent oxidative stress in the hypothalamus protects against obesity by modulating \u03b23-adrenergic mechanisms"],"prefix":"10.1172","volume":"2","author":[{"given":"Heinrich E.","family":"Lob","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York, 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