{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T07:02:17Z","timestamp":1790060537465,"version":"4.0.1"},"reference-count":73,"publisher":"American Society for Clinical Investigation","issue":"14","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,7,20]]},"abstract":"<jats:p>\n                    Increasing NAD\n                    <jats:sup>+<\/jats:sup>\n                    levels by supplementing with the precursor nicotinamide mononucleotide (NMN) improves cardiac function in multiple mouse models of disease. While NMN influences several aspects of mitochondrial metabolism, the molecular mechanisms by which increased NAD\n                    <jats:sup>+<\/jats:sup>\n                    enhances cardiac function are poorly understood. A putative mechanism of NAD\n                    <jats:sup>+<\/jats:sup>\n                    therapeutic action exists via activation of the mitochondrial NAD\n                    <jats:sup>+<\/jats:sup>\n                    -dependent protein deacetylase sirtuin 3 (SIRT3). We assessed the therapeutic efficacy of NMN and the role of SIRT3 in the Friedreich\u2019s ataxia cardiomyopathy mouse model (FXN-KO). At baseline, the FXN-KO heart has mitochondrial protein hyperacetylation, reduced\n                    <jats:italic>Sirt3<\/jats:italic>\n                    mRNA expression, and evidence of increased NAD\n                    <jats:sup>+<\/jats:sup>\n                    salvage. Remarkably, NMN administered to FXN-KO mice restores cardiac function to near-normal levels. To determine whether SIRT3 is required for NMN therapeutic efficacy, we generated SIRT3-KO and SIRT3-KO\/FXN-KO (double KO [dKO]) models. The improvement in cardiac function upon NMN treatment in the FXN-KO is lost in the dKO model, demonstrating that the effects of NMN are dependent upon cardiac SIRT3. Coupled with cardio-protection, SIRT3 mediates NMN-induced improvements in both cardiac and extracardiac metabolic function and energy metabolism. Taken together, these results serve as important preclinical data for NMN supplementation or SIRT3 activator therapy in Friedreich\u2019s ataxia patients.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.93885","type":"journal-article","created":{"date-parts":[[2017,7,19]],"date-time":"2017-07-19T11:22:28Z","timestamp":1500463348000},"source":"Crossref","is-referenced-by-count":126,"title":["Nicotinamide mononucleotide requires SIRT3 to improve cardiac function and bioenergetics in a Friedreich\u2019s ataxia cardiomyopathy model"],"prefix":"10.1172","volume":"2","author":[{"given":"Angelical S.","family":"Martin","sequence":"first","affiliation":[{"name":"Duke Molecular Physiology Institute and Sarah W. 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