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Changes in gene expression were confirmed by real-time PCR (RT-PCR) in 36 DCM and 21 NF pediatric hearts and in 20 DCM and 10 NF adult hearts. The degree of myocyte hypertrophy was investigated in 4 DCM and 7 NF pediatric hearts and in 4 DCM and 9 NF adult hearts. Changes in gene expression in response to pluripotency-inducing factors were investigated in neonatal rat ventricular myocytes (NRVMs). Transcriptome analysis identified a gene expression profile in children compared with adults with DCM. Additionally, myocyte hypertrophy was not observed in pediatric hearts but was present in adult hearts. Furthermore, treatment of NRVMs with pluripotency-inducing factors recapitulated changes in gene expression observed in the pediatric DCM heart. Pediatric DCM is characterized by unique changes in gene expression that suggest maintenance of an undifferentiated state.<\/jats:p>","DOI":"10.1172\/jci.insight.94249","type":"journal-article","created":{"date-parts":[[2017,7,19]],"date-time":"2017-07-19T11:22:28Z","timestamp":1500463348000},"source":"Crossref","is-referenced-by-count":51,"title":["Pediatric dilated cardiomyopathy hearts display a unique gene expression profile"],"prefix":"10.1172","volume":"2","author":[{"given":"Philip D.","family":"Tatman","sequence":"first","affiliation":[{"name":"Division of Cardiology, University of Colorado School of Medicine, Aurora, Colorado, USA."},{"name":"Medical Scientist Training Program and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathleen C.","family":"Woulfe","sequence":"additional","affiliation":[{"name":"Division of Cardiology, University of Colorado School of Medicine, Aurora, Colorado, 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