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Considering the numerous effects of the F508del mutation on the assembly and processing of CFTR protein, combination therapy with several pharmacological correctors is likely to be required to treat CF patients. Recently, it has been reported that thymosin \u03b1-1 (T\u03b1-1) has multiple beneficial effects that could lead to a single-molecule-based therapy for CF patients with F508del. Such effects include suppression of inflammation, improvement in F508del-CFTR maturation and gating, and stimulation of chloride secretion through the calcium-activated chloride channel (CaCC). Given the importance of such a drug, we aimed to characterize the underlying molecular mechanisms of action of T\u03b1-1. In-depth analysis of T\u03b1-1 effects was performed using well-established microfluorimetric, biochemical, and electrophysiological techniques on epithelial cell lines and primary bronchial epithelial cells from CF patients. The studies, which were conducted in 2 independent laboratories with identical outcome, demonstrated that T\u03b1-1 is devoid of activity on mutant CFTR as well as on CaCC. Although T\u03b1-1 may still be useful as an antiinflammatory agent, its ability to target defective anion transport in CF remains to be further investigated.<\/jats:p>","DOI":"10.1172\/jci.insight.98699","type":"journal-article","created":{"date-parts":[[2018,2,7]],"date-time":"2018-02-07T11:01:03Z","timestamp":1518001263000},"source":"Crossref","is-referenced-by-count":23,"title":["Thymosin \u03b1-1 does not correct F508del-CFTR in cystic fibrosis airway epithelia"],"prefix":"10.1172","volume":"3","author":[{"given":"Valeria","family":"Tomati","sequence":"first","affiliation":[{"name":"U.O.C. Genetica Medica, Istituto Giannina Gaslini, Genova, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emanuela","family":"Caci","sequence":"additional","affiliation":[{"name":"U.O.C. 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