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We hypothesized that water entry was mediated by the water channel aquaporin-1 (AQP1) and aimed to determine its role in the platelet procoagulant response and thrombosis. We established that human and mouse platelets express AQP1 and localize to internal tubular membrane structures. However, deletion of AQP1 had minimal effects on collagen-induced platelet granule secretion, aggregation, or membrane ballooning. Conversely, procoagulant spreading, microvesiculation, phosphatidylserine exposure, and clot formation time were significantly diminished. Furthermore, in vivo thrombus formation after FeCl\n                    <jats:sub>3<\/jats:sub>\n                    injury to carotid arteries was also markedly suppressed in AQP1-null mice, but hemostasis after tail bleeding remained normal. The mechanism involves an AQP1-mediated rapid membrane stretching during procoagulant spreading but not ballooning, leading to calcium entry through mechanosensitive cation channels and a full procoagulant response. We conclude that AQP1 is a major regulator of the platelet procoagulant response, able to modulate coagulation after injury or pathologic stimuli without affecting other platelet functional responses or normal hemostasis. Clinically effective AQP1 inhibitors may therefore represent a novel class of antiprocoagulant antithrombotics.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.99062","type":"journal-article","created":{"date-parts":[[2018,5,16]],"date-time":"2018-05-16T11:00:54Z","timestamp":1526468454000},"source":"Crossref","is-referenced-by-count":38,"title":["Aquaporin-1 regulates platelet procoagulant membrane dynamics and in vivo thrombosis"],"prefix":"10.1172","volume":"3","author":[{"given":"Ejaife O.","family":"Agbani","sequence":"first","affiliation":[{"name":"School of Physiology, Pharmacology and Neuroscience, University of Bristol, Biomedical Sciences Building, Bristol, United Kingdom."},{"name":"Department of Physiology and Pharmacology, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher M.","family":"Williams","sequence":"additional","affiliation":[{"name":"School of 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