{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T13:13:55Z","timestamp":1779196435617,"version":"3.51.4"},"reference-count":86,"publisher":"American Society for Clinical Investigation","issue":"23","funder":[{"name":"McNair Medical Institute Foundation","award":["McNair Scholar to Massimo Pietropaolo"],"award-info":[{"award-number":["McNair Scholar to Massimo Pietropaolo"]}]},{"DOI":"10.13039\/100007270","name":"University of Michigan","doi-asserted-by":"crossref","award":["Targeting Killer"],"award-info":[{"award-number":["Targeting Killer"]}],"id":[{"id":"10.13039\/100007270","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100007270","name":"University of Michigan","doi-asserted-by":"crossref","award":["Regulatory B Lymphocyte Function in IDDM"],"award-info":[{"award-number":["Regulatory B Lymphocyte Function in IDDM"]}],"id":[{"id":"10.13039\/100007270","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,12,6]]},"abstract":"<jats:p>\n                    We describe a protective effect on autoimmune diabetes and reduced destructive insulitis in NOD.\n                    <jats:italic>scid<\/jats:italic>\n                    recipients following splenocyte injections from diabetic NOD donors and sorted CD19\n                    <jats:sup>+<\/jats:sup>\n                    cells compared with NOD.\n                    <jats:italic>scid<\/jats:italic>\n                    recipients receiving splenocytes alone. This protective effect was age specific (only CD19\n                    <jats:sup>+<\/jats:sup>\n                    cells from young NOD donors exerted this effect;\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.001). We found that the CD19\n                    <jats:sup>+<\/jats:sup>\n                    IgM\n                    <jats:sup>+<\/jats:sup>\n                    cell is the primary subpopulation of B cells that delayed transfer of diabetes mediated by diabetogenic T cells from NOD mice (\n                    <jats:italic>P<\/jats:italic>\n                    = 0.002). Removal of IgM\n                    <jats:sup>+<\/jats:sup>\n                    cells from the CD19\n                    <jats:sup>+<\/jats:sup>\n                    pool did not result in protection. Notably, protection conferred by CD19\n                    <jats:sup>+<\/jats:sup>\n                    IgM\n                    <jats:sup>+<\/jats:sup>\n                    cotransfers were not dependent on the presence of Tregs, as their depletion did not affect their ability to delay onset of diabetes. Blockade of IL-10 with neutralizing antibodies at the time of CD19\n                    <jats:sup>+<\/jats:sup>\n                    cell cotransfers also abrogated the therapeutic effect, suggesting that IL-10 secretion was an important component of protection. These results were strengthened by ex vivo incubation of CD19\n                    <jats:sup>+<\/jats:sup>\n                    cells with IL-5, resulting in enhanced proliferation and IL-10 production and equivalently delayed diabetes progression (\n                    <jats:italic>P<\/jats:italic>\n                    = 0.0005). The potential to expand CD19\n                    <jats:sup>+<\/jats:sup>\n                    IgM\n                    <jats:sup>+<\/jats:sup>\n                    cells, especially in response to IL-5 stimulation or by pharmacologic agents, may be a new therapeutic option for type 1 diabetes.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.99860","type":"journal-article","created":{"date-parts":[[2018,12,5]],"date-time":"2018-12-05T11:01:32Z","timestamp":1544007692000},"source":"Crossref","is-referenced-by-count":8,"title":["CD19+IgM+ cells demonstrate enhanced therapeutic efficacy in type 1 diabetes mellitus"],"prefix":"10.1172","volume":"3","author":[{"given":"Andrew D.","family":"Vonberg","sequence":"first","affiliation":[{"name":"Diabetes Research Center, Division of Diabetes, Endocrinology and Metabolism Department of Medicine, 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