{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T05:22:52Z","timestamp":1789104172674,"version":"build-2803163510"},"reference-count":34,"publisher":"American Society for Clinical Investigation","issue":"13","license":[{"start":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T00:00:00Z","timestamp":1719792000000},"content-version":"vor","delay-in-days":48,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003977","name":"Israel Science Foundation","doi-asserted-by":"crossref","award":["1385\/20"],"award-info":[{"award-number":["1385\/20"]}],"id":[{"id":"10.13039\/501100003977","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001674","name":"Fondation Leducq","doi-asserted-by":"crossref","award":["20CVD01"],"award-info":[{"award-number":["20CVD01"]}],"id":[{"id":"10.13039\/501100001674","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,7,1]]},"abstract":"<jats:p>\n                    Cardiomyocyte sarcomeres contain localized ribosomes, but the factors responsible for their localization and the significance of localized translation are unknown. Using proximity labeling, we identified ribosomal protein SA (RPSA) as a Z-line protein. In cultured cardiomyocytes, the loss of RPSA led to impaired local protein translation and reduced sarcomere integrity. By employing CAS9-expressing mice, along with adeno-associated viruses expressing CRE recombinase and single-guide RNAs targeting\n                    <jats:italic>Rpsa<\/jats:italic>\n                    , we knocked out\n                    <jats:italic>Rpsa<\/jats:italic>\n                    in vivo and observed mislocalization of ribosomes and diminished local translation. These genetic mosaic mice with\n                    <jats:italic>Rpsa<\/jats:italic>\n                    knockout in a subset of cardiomyocytes developed dilated cardiomyopathy, featuring atrophy of RPSA-deficient cardiomyocytes, compensatory hypertrophy of unaffected cardiomyocytes, left ventricular dilation, and impaired contractile function. We demonstrated that RPSA C-terminal domain is sufficient for localization to the Z-lines and that if the microtubule network is disrupted RPSA loses its sarcomeric localization. These findings highlight RPSA as a ribosomal factor essential for ribosome localization to the Z-line, facilitating local translation and sarcomere maintenance.\n                  <\/jats:p>","DOI":"10.1172\/jci174527","type":"journal-article","created":{"date-parts":[[2024,5,14]],"date-time":"2024-05-14T12:00:37Z","timestamp":1715688037000},"source":"Crossref","is-referenced-by-count":13,"title":["Localized translation and sarcomere maintenance requires ribosomal protein SA in mice"],"prefix":"10.1172","volume":"134","author":[{"given":"Rami","family":"Haddad","sequence":"first","affiliation":[{"name":"Rappaport Family Institute for Research in the Medical Sciences and The Ruth and Bruce Rappaport Faculty of Medicine,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omer","family":"Sadeh","sequence":"additional","affiliation":[{"name":"Rappaport Family Institute for Research in the Medical Sciences and The Ruth and Bruce Rappaport Faculty of Medicine,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamar","family":"Ziv","sequence":"additional","affiliation":[{"name":"Smoler Proteomics Center, Lorry I. 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