{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T10:04:04Z","timestamp":1792317844535,"version":"4.4.0"},"reference-count":65,"publisher":"American Society for Clinical Investigation","issue":"19","license":[{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"vor","delay-in-days":35,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key Research and Development Program of China","award":["2022YFA1106200"],"award-info":[{"award-number":["2022YFA1106200"]}]},{"name":"National Key Research and Development Program of China","award":["2021YFA1102700"],"award-info":[{"award-number":["2021YFA1102700"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82270307"],"award-info":[{"award-number":["82270307"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32200660"],"award-info":[{"award-number":["32200660"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"crossref","award":["2022A1515011325"],"award-info":[{"award-number":["2022A1515011325"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100016799","name":"Natural Science Foundation of Guangzhou City","doi-asserted-by":"crossref","award":["202201011012"],"award-info":[{"award-number":["202201011012"]}],"id":[{"id":"10.13039\/100016799","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"crossref","award":["2024B1515020058"],"award-info":[{"award-number":["2024B1515020058"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,10,1]]},"abstract":"<jats:p>\n                    Cardiac mononuclear phagocytic cells (Cardiac MPCs) participate in maintaining homeostasis and orchestrating cardiac responses upon injury. However, the function of specific MPC subtypes and the related cell fate commitment mechanisms remain elusive in regenerative and nonregenerative hearts due to their cellular heterogeneities. Using spatiotemporal single-cell epigenomic analysis of cardiac MPCs in regenerative (P1) and nonregenerative (P10) mouse hearts after injury, we found that P1 hearts accumulate reparative\n                    <jats:italic>Arg1<\/jats:italic>\n                    <jats:sup>+<\/jats:sup>\n                    macrophages, while proinflammatory\n                    <jats:italic>S100a9<\/jats:italic>\n                    <jats:sup>+<\/jats:sup>\n                    <jats:italic>Ly6c<\/jats:italic>\n                    <jats:sup>+<\/jats:sup>\n                    monocytes are uniquely abundant during nonregenerative remodeling. Moreover, blocking chemokine CXCR2 to inhibit the specification of the\n                    <jats:italic>S100a9<\/jats:italic>\n                    <jats:sup>+<\/jats:sup>\n                    <jats:italic>Ly6c<\/jats:italic>\n                    <jats:sup>+<\/jats:sup>\n                    -biased inflammatory fate in P10 hearts resulted in elevated wound repair responses and marked improvements in cardiac function after injury. Single-cell RNA-Seq further confirmed an increased\n                    <jats:italic>Arg1<\/jats:italic>\n                    <jats:sup>+<\/jats:sup>\n                    macrophage subpopulation after CXCR2 blockade, which was accomplished by increased expression of wound repair\u2013related genes and reduced expression of proinflammatory genes. Collectively, our findings provide instructive insights into the molecular mechanisms underlying the function and fate specification of heterogeneous MPCs during cardiac repair and identify potential therapeutic targets for myocardial infarction.\n                  <\/jats:p>","DOI":"10.1172\/jci175297","type":"journal-article","created":{"date-parts":[[2024,8,27]],"date-time":"2024-08-27T13:12:46Z","timestamp":1724764366000},"source":"Crossref","is-referenced-by-count":28,"title":["Single-cell multiomic analysis identifies macrophage subpopulations in promoting cardiac repair"],"prefix":"10.1172","volume":"134","author":[{"given":"Mingzhu","family":"Fu","sequence":"first","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Bone and Joint Degeneration Diseases, Department of Physiology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 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