{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T15:08:03Z","timestamp":1791299283342,"version":"4.1.0"},"reference-count":26,"publisher":"American Society for Clinical Investigation","issue":"9","license":[{"start":{"date-parts":[[2024,5,1]],"date-time":"2024-05-01T00:00:00Z","timestamp":1714521600000},"content-version":"vor","delay-in-days":50,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82000389"],"award-info":[{"award-number":["82000389"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"The project of Shanghai Municipal Science and Technology Commission","award":["20MC1920400"],"award-info":[{"award-number":["20MC1920400"]}]},{"name":"Shanghai Sailing Program","award":["20YF1428700"],"award-info":[{"award-number":["20YF1428700"]}]},{"name":"Shanghai Chenguang Program","award":["20CG23"],"award-info":[{"award-number":["20CG23"]}]},{"name":"Natural Sciences Foundation of Fujian","award":["2022J05105"],"award-info":[{"award-number":["2022J05105"]}]},{"DOI":"10.13039\/100020732","name":"Innovative research team of high-level local universities in Shanghai","doi-asserted-by":"crossref","award":["2021"],"award-info":[{"award-number":["2021"]}],"id":[{"id":"10.13039\/100020732","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Shanghai Research Center for Pediatric Cardiovascular Diseases","award":["2023ZZ02024"],"award-info":[{"award-number":["2023ZZ02024"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,5,1]]},"abstract":"<jats:p>\n                    Hypothermia is commonly used to protect donor hearts during transplantation. However, patients transplanted with aged donor hearts still have severe myocardial injury and decreased survival rates, but the underlying mechanism remains unknown. Because aged hearts are not considered suitable for donation, the number of patients awaiting heart transplants is increasing. In this study, we examined whether hypothermic cardioprotection was attenuated in aged donor hearts during transplantation and evaluated potential therapeutic targets. Using a rat heart transplantation model, we found that hypothermic cardioprotection was impaired in aged donor hearts but preserved in young donor hearts. RNA-Seq showed that cold-inducible RNA-binding protein (\n                    <jats:italic>Cirbp<\/jats:italic>\n                    ) expression was decreased in aged donor hearts, and these hearts showed severe ferroptosis after transplantation. The young donor hearts from\n                    <jats:italic>Cirbp<\/jats:italic>\n                    -KO rats exhibited attenuated hypothermic cardioprotection, but\n                    <jats:italic>Cirbp<\/jats:italic>\n                    overexpression in aged donor hearts ameliorated hypothermic cardioprotection. Cardiac proteomes revealed that dihydroorotate dehydrogenase (DHODH) expression was significantly decreased in\n                    <jats:italic>Cirbp<\/jats:italic>\n                    -KO donor hearts during transplantation. Consequently, DHODH-mediated ubiquinone reduction was compromised, thereby exacerbating cardiac lipid peroxidation and triggering ferroptosis after transplantation. A cardioplegic solution supplemented with CIRBP agonists improved hypothermic cardioprotection in aged donor hearts, indicating that this method has the potential to broaden the indications for using aged donor hearts in transplantation.\n                  <\/jats:p>","DOI":"10.1172\/jci175645","type":"journal-article","created":{"date-parts":[[2024,4,30]],"date-time":"2024-04-30T14:03:00Z","timestamp":1714485780000},"source":"Crossref","is-referenced-by-count":35,"title":["Cirbp suppression compromises DHODH-mediated ferroptosis defense and attenuates hypothermic cardioprotection in an aged donor transplantation model"],"prefix":"10.1172","volume":"134","author":[{"given":"Yifan","family":"Zhu","sequence":"first","affiliation":[{"name":"Heart Center and Shanghai Institute of Pediatric Congenital Heart Disease, Shanghai Children\u2019s Medical Center, National Children\u2019s Medical Center,"},{"name":"Department of Cardiothoracic Surgery, Shanghai Children\u2019s Medical Center, National Children\u2019s Medical Center, and"},{"name":"Shanghai Clinical Research Center for Rare Pediatric Diseases, Shanghai Children\u2019s Medical Center, National Children\u2019s Medical Center, Shanghai Jiaotong University School of Medicine, Shanghai, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenyu","family":"Jiang","sequence":"additional","affiliation":[{"name":"Heart Center and Shanghai Institute of Pediatric Congenital Heart Disease, Shanghai Children\u2019s Medical Center, National Children\u2019s Medical Center,"},{"name":"Department of Cardiothoracic Surgery, Shanghai Children\u2019s Medical Center, National Children\u2019s Medical Center, and"},{"name":"Shanghai Clinical Research Center for Rare Pediatric Diseases, Shanghai Children\u2019s Medical Center, National Children\u2019s Medical Center, Shanghai Jiaotong University School of Medicine, Shanghai, 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