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For this purpose, 2 patient cohorts were enrolled: patients with OvCa who underwent a diagnostic or cytoreductive surgery and nononcological patients, who underwent abdominal surgery for benign gynecological conditions and acted as the control group. Systematic extraction of PFD-sEVs from surgical samples enabled us to observe significant quantitative and qualitative differences associated with cancer diagnosis, disease stage, and platinum chemosensitivity. Proteomic profiling of PFD-sEVs led to the identification of molecular pathways and proteins of interest and to the biological validation of S100A4 and STX5. In addition, unsupervised analysis of PFD-sEV proteomic profiles in high-grade serous ovarian carcinomas (HGSOCs) revealed 2 clusters with different outcomes in terms of overall survival. In conclusion, comprehensive characterization of PFD-sEV content provided a prognostic value with potential implications in HGSOC clinical management.<\/jats:p>","DOI":"10.1172\/jci176161","type":"journal-article","created":{"date-parts":[[2024,4,2]],"date-time":"2024-04-02T12:00:17Z","timestamp":1712059217000},"source":"Crossref","is-referenced-by-count":31,"title":["Proteomic profiles of peritoneal fluid-derived small extracellular vesicles correlate with patient outcome in ovarian cancer"],"prefix":"10.1172","volume":"134","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9302-0905","authenticated-orcid":true,"given":"Miguel","family":"Quiralte","sequence":"first","affiliation":[{"name":"Laboratory of Innovation in Oncology, Clara Campal Comprehensive Cancer Centre (HM CIOCC), HM Sanchinarro University Hospital, Madrid, Spain."},{"name":"Institute of Applied Molecular Medicine, Faculty of Medicine, Universidad San Pablo\u2013CEU, Alcorc\u00f3n, Madrid, 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