{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T22:19:36Z","timestamp":1793657976265,"version":"4.5.1"},"reference-count":82,"publisher":"American Society for Clinical Investigation","issue":"18","license":[{"start":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T00:00:00Z","timestamp":1757980800000},"content-version":"vor","delay-in-days":68,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100017540","name":"NHLBI Division of Intramural Research","doi-asserted-by":"crossref","award":["R35HL177401"],"award-info":[{"award-number":["R35HL177401"]}],"id":[{"id":"10.13039\/100017540","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,9,16]]},"abstract":"<jats:p>\n                    The role of conventional type 1 DCs (cDC1s) in tolerance induction to solid organ allografts is unknown and important for strategies that seek to prolong allograft viability. Using a murine model deficient in cDC1s, we report cDC1s are required for donor antigen and costimulation blockade (DST + CoB) tolerance induction and survival of cardiac allografts. cDC1 deficiency led to decreases in CD4\n                    <jats:sup>+<\/jats:sup>\n                    CD25\n                    <jats:sup>+<\/jats:sup>\n                    FoxP3\n                    <jats:sup>+<\/jats:sup>\n                    T cells within allograft and spleen tissue of transplant recipients, and this was found to be antigen specific. Donor antigen stimulation induced TGF-\u03b21 expression in both in vivo cDC1s and in vitro Flt3L-derived cDC1s. Genetic deletion of\n                    <jats:italic>TGF-<\/jats:italic>\n                    <jats:italic>\u03b2<\/jats:italic>\n                    <jats:italic>1<\/jats:italic>\n                    in cDC1s prevented induction of antigen-specific CD4\n                    <jats:sup>+<\/jats:sup>\n                    CD25\n                    <jats:sup>+<\/jats:sup>\n                    FoxP3\n                    <jats:sup>+<\/jats:sup>\n                    T cells and was associated with cardiac allograft rejection. In parallel, single-cell RNA sequencing and metabolic analysis revealed upregulation of cDC1 mitochondrial metabolic signatures after in vivo exposure to DST + CoB. Genetic inactivation of cDC1 mitochondrial metabolism reduced expression of cDC1 TGF-\u03b21, decreased antigen-specific Treg populations, and impaired allograft tolerance. Taken together, our findings implicate cDC1s in strategies to preserve solid organ allografts and also implicate mitochondrial metabolism of cDC1s as a molecular mechanism to enhance the generation of antigen-specific CD4\n                    <jats:sup>+<\/jats:sup>\n                    CD25\n                    <jats:sup>+<\/jats:sup>\n                    FoxP3\n                    <jats:sup>+<\/jats:sup>\n                    T cells through TGF-\u03b21.\n                  <\/jats:p>","DOI":"10.1172\/jci178960","type":"journal-article","created":{"date-parts":[[2025,7,11]],"date-time":"2025-07-11T17:39:27Z","timestamp":1752255567000},"source":"Crossref","is-referenced-by-count":9,"title":["Treg activation during allograft tolerance induction requires mitochondrion-induced TGF-\u03b21 in type 1 conventional dendritic cells"],"prefix":"10.1172","volume":"135","author":[{"given":"Samantha L.","family":"Schroth","sequence":"first","affiliation":[{"name":"Northwestern University Feinberg 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