{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T14:04:41Z","timestamp":1791813881759,"version":"4.3.4"},"reference-count":55,"publisher":"American Society for Clinical Investigation","issue":"22","license":[{"start":{"date-parts":[[2024,11,15]],"date-time":"2024-11-15T00:00:00Z","timestamp":1731628800000},"content-version":"vor","delay-in-days":57,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["HL160610"],"award-info":[{"award-number":["HL160610"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"name":"University Award, University of Rochester","award":["UA Pilot Program"],"award-info":[{"award-number":["UA Pilot Program"]}]},{"name":"a","award":["b"],"award-info":[{"award-number":["b"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,11,15]]},"abstract":"<jats:p>\n                    Lung megakaryocytes (Mks) are largely extravascular with an immune phenotype (1). Because bone marrow (BM) Mks are short lived, it has been assumed that extravascular lung Mks are constantly \u201cseeded\u201d from the BM. To investigate lung Mk origins and how origin affects their functions, we developed methods to specifically label lung Mks using CFSE dye and biotin delivered via the oropharyngeal route. Labeled lung Mks were present for up to 4 months, while BM Mks had a lifespan of less than 1 week. In a parabiosis model, lung Mks were partially replaced over 1 month from a circulating source. Unlike tissue-resident macrophages, using MDS1\n                    <jats:sup>-Cre-ERT2<\/jats:sup>\n                    TdTomato mice, we found that lung Mks arose from hematopoietic stem cells. However, studies with FlkSwitch mTmG mice showed that lung Mks were derived from a Flt3-independent lineage that did not go through a multipotent progenitor. CFSE labeling to track lung Mk\u2013derived platelets showed that approximately 10% of circulating platelets were derived from lung-resident Mks at steady state, but in sterile thrombocytopenia this was doubled (~20%). Lung-derived platelets were similarly increased in a malaria infection model (\n                    <jats:italic>Plasmodium yoelii<\/jats:italic>\n                    ) typified by thrombocytopenia. These studies indicate that lung Mks arise from a Flt3\n                    <jats:sup>\u2013<\/jats:sup>\n                    BM source, are long-lived, and contribute more platelets during thrombocytopenia.\n                  <\/jats:p>","DOI":"10.1172\/jci181111","type":"journal-article","created":{"date-parts":[[2024,9,20]],"date-time":"2024-09-20T11:29:18Z","timestamp":1726831758000},"source":"Crossref","is-referenced-by-count":35,"title":["Long-lived lung megakaryocytes contribute to platelet recovery in thrombocytopenia models"],"prefix":"10.1172","volume":"134","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2886-8819","authenticated-orcid":true,"given":"Alison C.","family":"Livada","sequence":"first","affiliation":[{"name":"Aab Cardiovascular Research Institute,"},{"name":"Department of Pathology and Laboratory Medicine,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2372-6109","authenticated-orcid":true,"given":"Kathleen 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