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We have used automated and nasogastric feeding to address this question in male rodents and human volunteers. While grazing and meal-feeding reduced food intake in parallel (compared with ad libitum\u2013fed rodents), body length and tibial epiphysial plate width were maintained in meal-fed rodents via the action of ghrelin and its receptor, GHS-R. Grazing and meal-feeding initially suppressed elevated preprandial ghrelin levels in rats, followed by either a sustained elevation in ghrelin in grazing rats or preprandial ghrelin surges in meal-fed rats. Episodic growth hormone (GH) secretion was largely unaffected in grazing rats, but meal-feeding tripled GH secretion, with burst height augmented and 2 additional bursts of GH per day. Continuous nasogastric infusion of enteral feed in humans failed to suppress circulating ghrelin, producing continuously elevated circulating GH levels with minimal rhythmicity. In contrast, bolus enteral infusion elicited postprandial ghrelin troughs accompanied by reduced circulating GH, with enhanced ultradian rhythmicity. Taken together, our data imply that the contemporary shift from regular meals to snacking behavior may be detrimental to optimal skeletal growth outcomes by sustaining circulating ghrelin at levels associated with undernourishment and diminishing GH pulsatility.<\/jats:p>","DOI":"10.1172\/jci189202","type":"journal-article","created":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T12:01:15Z","timestamp":1743508875000},"source":"Crossref","is-referenced-by-count":4,"title":["Meal-feeding promotes skeletal growth by ghrelin-dependent enhancement of growth hormone rhythmicity"],"prefix":"10.1172","volume":"135","author":[{"given":"Amanda K.E.","family":"Hornsby","sequence":"first","affiliation":[{"name":"School of Biosciences, Cardiff University, Cardiff, United Kingdom."},{"name":"Institute of Life Sciences, School of Medicine, Swansea University, Swansea, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard 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