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SS18 participates in BAF complexes that open chromatin, while the SSX genes are cancer-testis antigens that interface with chromatin decorated with monoubiquitinated histone H2A placed by polycomb repressive complex activity. Because KDM2B brings polycomb repressive complex to unmethylated CpG islands, it is plausible that methylation directly determines the distribution of SS18::SSX to target loci. Given that synovial sarcoma is also characterized by a peculiarly low DNA hypomethylation profile, we hypothesized that further disturbance of DNA methylation would have a negative impact on synovial sarcoma growth. DNMT1 disruption by CRISPR\/Cas9 targeting or pharmacological inhibition with cytidine analogs 5-aza-2\u2032-deoxycytidine (decitabine) and 5-azacytidine led to decreased genome-wide methylation, redistribution of SS18::SSX, and altered gene expression profiles, most prominently including upregulation of tumor suppressor genes, immune-related genes, and mesenchymal differentiation-related genes. These drugs suppressed growth of synovial sarcoma cell lines and drove cytoreduction in mouse genetic models. DNMT1 inhibitors, already approved for treating myelodysplastic syndromes, warrant further clinical investigation for synovial sarcoma as repurposed, targeted treatments exploiting a vulnerability in the intrinsic biology of this cancer.<\/jats:p>","DOI":"10.1172\/jci190855","type":"journal-article","created":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T12:00:17Z","timestamp":1745928017000},"source":"Crossref","is-referenced-by-count":8,"title":["DNA demethylating agents suppress preclinical models of synovial sarcoma"],"prefix":"10.1172","volume":"135","author":[{"given":"Nobuhiko","family":"Hasegawa","sequence":"first","affiliation":[{"name":"Department of Orthopaedics,"},{"name":"Department of Oncological Sciences,"},{"name":"Huntsman Cancer Institute, University of Utah, Salt Lake City, Utah, USA."},{"name":"Department of Orthopaedics, Juntendo University, Faculty of Medicine, Tokyo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nezha 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