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In a cohort of more than 1,000 patients with BC, we identified SH3BP5L as the most highly expressed guanine nucleotide exchange factor (GEF) for RAB11A. High SH3BP5L expression marked an advanced tumor stage, distant metastasis, and poor prognosis, with significant associations in human epidermal growth factor receptor 2\u2013positive (HER2\n                    <jats:sup>\n                      <jats:sup>+<\/jats:sup>\n                    <\/jats:sup>\n                    ) and triple-negative breast cancer (TNBC). Using F\u00f6rster resonance energy transfer (FRET) sensors and artificial intelligence\u2013 (AI-assisted) microscopy, we showed that cargo delivery to the plasma membrane required SH3BP5L-dependent activation of RAB11A and assembly of a complex with the anterograde motor KIF5B. This trafficking governed key metastatic features of TNBC, including \u03b21 integrin recycling and \u03b13\u03b21 integrin surface exposure. 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