{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T04:43:35Z","timestamp":1791607415935,"version":"4.3.4"},"reference-count":62,"publisher":"American Society for Clinical Investigation","issue":"22","license":[{"start":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T00:00:00Z","timestamp":1763337600000},"content-version":"vor","delay-in-days":62,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["F32HL162418,T32GM144295,T32HL076139,F31HL162490,T32AI083216,K08HL159356,U19AI135964,R01HL149883,R01HL153122,P01HL154998,P01AG049665,U19AI181102"],"award-info":[{"award-number":["F32HL162418,T32GM144295,T32HL076139,F31HL162490,T32AI083216,K08HL159356,U19AI135964,R01HL149883,R01HL153122,P01HL154998,P01AG049665,U19AI181102"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,11,17]]},"abstract":"<jats:p>\n                    FOXP3\n                    <jats:sup>+<\/jats:sup>\n                    natural regulatory T cells (nTregs) promote resolution of inflammation and repair of epithelial damage following viral pneumonia\u2013induced lung injury, thus representing a cellular therapy for patients with severe viral pneumonia and the acute respiratory distress syndrome. Whether in vitro\u2013induced Tregs (iTregs), which can be rapidly generated in substantial numbers from conventional T cells, also promote lung recovery is unknown. nTregs require specific DNA methylation patterns maintained by the epigenetic regulator ubiquitin-like with PHD and RING finger domains 1 (UHRF1). Here, we tested whether iTregs promote recovery following viral pneumonia and whether iTregs require UHRF1 for their pro-recovery function. We found that adoptive transfer of iTregs to mice with influenza virus pneumonia promotes lung recovery and that loss of UHRF1-mediated maintenance DNA methylation in iTregs leads to reduced engraftment and a delayed repair response. Transcriptional and DNA methylation profiling of adoptively transferred UHRF1-deficient iTregs that had trafficked to influenza-injured lungs demonstrated transcriptional instability with gain of transcription factors that define effector T cell lineage. Strategies to promote the stability of iTregs could be leveraged to further augment their pro-recovery function during viral pneumonia and other causes of severe lung injury.\n                  <\/jats:p>","DOI":"10.1172\/jci192925","type":"journal-article","created":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T16:00:32Z","timestamp":1758038432000},"source":"Crossref","is-referenced-by-count":10,"title":["Maintenance DNA methylation is required for induced Treg reparative function following viral pneumonia in mice"],"prefix":"10.1172","volume":"135","author":[{"given":"Anthony M.","family":"Joudi","sequence":"first","affiliation":[{"name":"Division of Pulmonary and Critical Care Medicine,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan K.","family":"Gurkan","sequence":"additional","affiliation":[{"name":"Division of Pulmonary and Critical Care Medicine,"},{"name":"Medical Scientist Training Program,"},{"name":"Driskill Graduate 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