{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T21:53:04Z","timestamp":1791496384710,"version":"4.3.4"},"reference-count":53,"publisher":"American Society for Clinical Investigation","issue":"4","license":[{"start":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T00:00:00Z","timestamp":1771200000000},"content-version":"vor","delay-in-days":88,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000925","name":"NHMRC","doi-asserted-by":"crossref","award":["1149976"],"award-info":[{"award-number":["1149976"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000925","name":"NHMRC","doi-asserted-by":"crossref","award":["Investigator grant 2007839"],"award-info":[{"award-number":["Investigator grant 2007839"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000925","name":"NHMRC","doi-asserted-by":"crossref","award":["Investigator grant 2009476"],"award-info":[{"award-number":["Investigator grant 2009476"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000925","name":"NHMRC","doi-asserted-by":"crossref","award":["Investigator grant 2033228"],"award-info":[{"award-number":["Investigator grant 2033228"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,2,16]]},"abstract":"<jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>Checkpoint inhibitor\u2013associated autoimmune diabetes mellitus (CIADM) is a rare but life-altering complication of immune checkpoint inhibitor (ICI) therapy. Biomarkers that predict type 1 diabetes (T1D) are unreliable for CIADM.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>AIM<\/jats:title>\n                    <jats:p>In the present study, we sought to identify biomarkers for the prediction of CIADM.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS<\/jats:title>\n                    <jats:p>From our prospective biobank, we identified 14 patients with CIADM who had metastatic melanoma treated with anti\u2013programed antibody death 1 (anti\u2013PD-1) with or without anti\u2013cytotoxic T lymphocyte\u2013associated antibody protein 4 (anti-CTLA4). Controls were selected from the same biobank, matched 2:1. Pretreatment, on-ICI, and post-CIADM serum and PBMCs were analyzed. Serum was analyzed for T1D autoantibodies, C-peptide, glucose, and cytokines. PBMCs were profiled using flow cytometry. Pancreatic volume was measured using CT volumetry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      Before treatment, patients with CIADM had smaller pancreatic volume (27% reduction,\n                      <jats:italic>P<\/jats:italic>\n                      = 0.044) and higher anti\u2013glutamic acid decarboxylase autoantibody (anti-GAD) titers (median 2.9 vs. 0,\n                      <jats:italic>P<\/jats:italic>\n                      = 0.01). They had significantly higher baseline proportions of Th17 cells (\n                      <jats:italic>P<\/jats:italic>\n                      = 0.03), higher CD4\n                      <jats:sup>+<\/jats:sup>\n                      central memory cells (\n                      <jats:italic>P<\/jats:italic>\n                      = 0.04), and lower naive CD4\n                      <jats:sup>+<\/jats:sup>\n                      T cells (\n                      <jats:italic>P<\/jats:italic>\n                      = 0.01). With ICI treatment, greater declines in pancreatic volume were seen in patients with CIADM (\n                      <jats:italic>P<\/jats:italic>\n                      &lt; 0.0001). Activated CD4\n                      <jats:sup>+<\/jats:sup>\n                      T cell subsets increased significantly in CIADM and controls with immune-related adverse effects (IRAEs) but not in controls without IRAEs. Using only pretreatment results, we found that pancreatic volume, anti-GAD antibody titers, and the baseline immune flow profile were highly predictive of CIADM development, with an AUC of greater than 0.96.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSIONS<\/jats:title>\n                    <jats:p>People who develop CIADM are immunologically predisposed and have antecedent pancreatic and immunological changes that accurately predict disease with excellent sensitivity. These biomarkers could be used to guide ICI use, particularly when planning treatment for low-risk tumors.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>FUNDING<\/jats:title>\n                    <jats:p>National Health and Medical Research Council (NHMRC) Investigator grants 2033228, 2009476, and 2007839.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1172\/jci192938","type":"journal-article","created":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T17:00:16Z","timestamp":1763658016000},"source":"Crossref","is-referenced-by-count":6,"title":["Pancreatic volume and immune biomarkers predict checkpoint inhibitor\u2013associated autoimmune diabetes in humans"],"prefix":"10.1172","volume":"136","author":[{"given":"Linda","family":"Wu","sequence":"first","affiliation":[{"name":"Centre for Diabetes, Obesity and Endocrinology Research, Westmead Institute for Medical Research, Westmead, NSW, Sydney, 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