{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:59:32Z","timestamp":1787018372137,"version":"build-2736575974"},"reference-count":44,"publisher":"American Society for Clinical Investigation","issue":"20","license":[{"start":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T00:00:00Z","timestamp":1760486400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China for Excellent Young Scholars","award":["82022019"],"award-info":[{"award-number":["82022019"]}]},{"name":"The Innovation and Entrepreneurial Team of Sichuan Tianfu Emei Program","award":["CZ2024018"],"award-info":[{"award-number":["CZ2024018"]}]},{"name":"Funding for Distinguished Young Scholars of Sichuan Provincial People\u2019s Hospital","award":["30420230005"],"award-info":[{"award-number":["30420230005"]}]},{"name":"Funding for Distinguished Young Scholars of University of Electronic Science and Technology of China","award":["A1098531023601381"],"award-info":[{"award-number":["A1098531023601381"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["U24A20692,82371355,82101414"],"award-info":[{"award-number":["U24A20692,82371355,82101414"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,10,15]]},"abstract":"<jats:p>\n                    Multiple sclerosis (MS) is a progressive, chronic, and highly disabling neuroinflammatory disorder characterized by demyelination and T cell\u2013driven inflammation. Pathogenic T cells play a central role in MS, but effective therapeutic targeting remains challenging. Here, we identified ankyrin repeat domain\u2013containing protein 55 (ANKRD55) as a key regulator of T cell function by single-cell transcriptomic analysis of cerebrospinal fluid and blood from MS patients.\n                    <jats:italic>ANKRD55<\/jats:italic>\n                    was predominantly expressed in CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells in both compartments. Genetic ablation of\n                    <jats:italic>Ankrd55<\/jats:italic>\n                    led to a robustly reduced disease severity and neuroinflammation in experimental autoimmune encephalomyelitis (EAE), a widely used animal model for MS. Furthermore, T cell\u2013specific deficiency of\n                    <jats:italic>Ankrd55<\/jats:italic>\n                    significantly impaired Th1 polarization and Th17 differentiation, reducing EAE pathogenicity. Mechanistically, we found that\n                    <jats:italic>Ankrd55<\/jats:italic>\n                    deficiency disrupted T cell receptor (TCR) signaling integrity. We demonstrated that ANKRD55 regulates the formation of the immune synapse, an essential prerequisite for TCR activation, by interacting with subunits of the chaperonin-containing TCP1 (CCT) complex and modulating its activity, enhancing its assembly by competing with CCT5 for binding to TCP1, CCT3, and CCT6. This facilitates proper microtubule organization and TCR activation. These findings establish ANKRD55 as a critical regulator of TCR signaling and highlight its therapeutic potential in pathogenic T cell\u2013driven autoimmune diseases.\n                  <\/jats:p>","DOI":"10.1172\/jci195214","type":"journal-article","created":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T19:53:39Z","timestamp":1760471619000},"source":"Crossref","is-referenced-by-count":2,"title":["ANKRD55 is a key regulator of T cell inflammation in multiple sclerosis"],"prefix":"10.1172","volume":"135","author":[{"given":"Chuyu","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Neurology, Sichuan Provincial People\u2019s Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meiling","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Science and Technology, Sichuan Provincial People\u2019s Hospital, University of 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