{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T17:36:07Z","timestamp":1781285767799,"version":"3.54.1"},"reference-count":72,"publisher":"American Society for Clinical Investigation","issue":"6","license":[{"start":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T00:00:00Z","timestamp":1773619200000},"content-version":"vor","delay-in-days":60,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Programme for Research in Epidemic Preparedness And REsponse","award":["PREPARE-CS1-2022-002"],"award-info":[{"award-number":["PREPARE-CS1-2022-002"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,3,16]]},"abstract":"<jats:p>\n                    Short-lived, clade-specific immune responses with limited mucosal priming are limitations of current COVID-19 mRNA vaccines. We have developed a nasal booster vaccine candidate that induced robust, sustained, cross-clade, systemic, and mucosal protective immunity. Two recombinant Clec9A-specific monoclonal antibodies fused to the receptor binding domain (RBD) from Omicron XBB.1.5 and SARS-CoV-1 were generated. In Comirnaty mRNA\u2013vaccinated mice, boosting with both constructs combined (Clec9A\n                    <jats:sup>OMNI<\/jats:sup>\n                    ) induced cross-clade neutralizing antibodies and T cell responses that were greater in magnitude and more sustained compared with bivalent Comirnaty (BC) mRNA vaccine booster. Persistence of RBD-specific follicular helper CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells, germinal center B cells, and long-lived plasma cells that facilitated affinity maturation correlated with detection of triple cross-reactive B cells binding the RBDs of ancestral SARS-CoV-2, XBB.1.5, and SARS-CoV-1. Remarkably, intranasal boosting with Clec9A\n                    <jats:sup>OMNI<\/jats:sup>\n                    elicited robust and durable immunity across the upper and lower airways while concurrently boosting the systemic immunity to levels matching or exceeding those from systemic boosting. Correspondingly, Clec9A\n                    <jats:sup>OMNI<\/jats:sup>\n                    nasal booster conferred superior protection against SARS-CoV-2 challenge compared with BC mRNA booster, with undetectable viral titers in the respiratory tract. Hence, Clec9A\n                    <jats:sup>OMNI<\/jats:sup>\n                    is a promising nasal booster vaccine candidate that has the potential to mitigate pandemic threats from emerging sarbecoviruses.\n                  <\/jats:p>","DOI":"10.1172\/jci195784","type":"journal-article","created":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T17:00:40Z","timestamp":1768496440000},"source":"Crossref","is-referenced-by-count":3,"title":["Intranasal DC-targeting vaccine booster elicits durable and cross-clade protective immunity against sarbecoviruses in mice"],"prefix":"10.1172","volume":"136","author":[{"given":"Nicholas You Zhi","family":"Cheang","sequence":"first","affiliation":[{"name":"Infectious Diseases Translational Research Programme, Department of Microbiology and Immunology, Yong Loo Lin School of Medicine and"},{"name":"Immunology Programme, Life Sciences Institute, National University of Singapore, 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