{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T20:50:40Z","timestamp":1785876640151,"version":"3.56.0"},"reference-count":65,"publisher":"American Society for Clinical Investigation","issue":"9","license":[{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82472041,82071978,52271196"],"award-info":[{"award-number":["82472041,82071978,52271196"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Cancer Institute","award":["R01 CA212379"],"award-info":[{"award-number":["R01 CA212379"]}]},{"name":"National Cancer Center","award":["P30 CA008748"],"award-info":[{"award-number":["P30 CA008748"]}]},{"name":"Guangdong Provincial Joint Fund for Corporate Innovation and Development","award":["2024A1515220058"],"award-info":[{"award-number":["2024A1515220058"]}]},{"name":"National Key Research and Development Program of China","award":["2021YFF1200700"],"award-info":[{"award-number":["2021YFF1200700"]}]},{"name":"Young Talents Program of Sun Yat-sen University Cancer Center","award":["YTP-SYSUCC-0024"],"award-info":[{"award-number":["YTP-SYSUCC-0024"]}]},{"name":"Fundamental Research Funds for the Central Universities at Sun Yat-sen University","award":["31610026"],"award-info":[{"award-number":["31610026"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,5,1]]},"abstract":"<jats:p>Integrative multiscale imaging bridges the gap between macroscopic organ structures and microscopic cellular processes, enabling holistic visualization of anatomy and function across scales. Photoacoustic imaging (PAI) leverages melanin\u2019s potent contrast for label-free melanoma detection, yet its potential in lung imaging, challenged by air-tissue acoustic impedance mismatch, remains unexplored for melanoma lung metastases (MLMs). We used hierarchical multiscale PAI, transitioning from whole-body macroscale to localized mesoscale and single-cell-resolution microscale. PAI also guided photoablation interventions in the first and second near-infrared windows, requiring only 10.4 pg intracellular melanin\/cell. Bioinformatic analysis of human MLM tissues revealed perturbed signaling pathways compared with normal skin and lung tissues, accounting for dysfunctional melanogenesis to enable label-free PAI with high sensitivity and specificity. Malignant MLM lesions in living mice, resected mouse lungs, and human lungs were delineated with margins closely conforming to histology. The high sensitivity allowed visualization of low-cellularity microsatellite foci down to a few tens of cell clusters, with sufficient penetration in the lungs of mice and Bama minipigs. The multiscale imaging methodology streamlines a theranostic workflow and specifically identifies MLM burden in a progressive, label-free manner, which may aid real-time tumor ablation in the future.<\/jats:p>","DOI":"10.1172\/jci196095","type":"journal-article","created":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T18:00:58Z","timestamp":1777572058000},"source":"Crossref","is-referenced-by-count":1,"title":["Label-free streamlined photoacoustic image guidance facilitates NIR-II photoablation in models of melanoma lung metastases"],"prefix":"10.1172","volume":"136","author":[{"given":"Wei","family":"Xing","sequence":"first","affiliation":[{"name":"State Key Laboratory of Oncology in South China, Guangdong Provincial Clinical Research Center for Cancer, and"},{"name":"Department of Anesthesiology, Sun Yat-sen University Cancer Center, Guangzhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujia","family":"Zhou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oncology in South China, Guangdong Provincial Clinical Research Center for Cancer, and"},{"name":"Department of Cardiology, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katja","family":"Haedicke","sequence":"additional","affiliation":[{"name":"Department of Radiology, Memorial Sloan Kettering Cancer Center, New York, New York, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenyixin","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oncology in South China, Guangdong Provincial Clinical Research Center for Cancer, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karla Ximena","family":"Vazquez-Prada","sequence":"additional","affiliation":[{"name":"Department of Radiology, Memorial Sloan Kettering Cancer Center, New York, New York, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Wu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oncology in South China, Guangdong Provincial Clinical Research Center for Cancer, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhijun","family":"Lin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oncology in South China, Guangdong Provincial Clinical Research Center for Cancer, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chrysafis","family":"Andreou","sequence":"additional","affiliation":[{"name":"Department of Radiology, Memorial Sloan Kettering Cancer Center, New York, New York, USA."},{"name":"Department of Electrical and Computer Engineering, University of Cyprus, Nicosia, Cyprus."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qize","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Radiology, Memorial Sloan Kettering Cancer Center, New York, New York, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Shang","sequence":"additional","affiliation":[{"name":"Department of Oncology, Xinyang Central Hospital, Xinyang, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruoyang","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Oncology, Xinyang Central Hospital, Xinyang, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moritz","family":"Kircher","sequence":"additional","affiliation":[{"name":"Department of Radiology, Memorial Sloan Kettering Cancer Center, New York, New York, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingdong","family":"Ye","sequence":"additional","affiliation":[{"name":"Institute of Dermatology, Guangzhou Dermatology Hospital, Guangzhou Medical University, Guangzhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Grimm","sequence":"additional","affiliation":[{"name":"Department of Radiology, Memorial Sloan Kettering Cancer Center, New York, New York, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiang","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oncology in South China, Guangdong Provincial Clinical Research Center for Cancer, and"},{"name":"Department of Radiology, Memorial Sloan Kettering Cancer Center, New York, New York, USA."},{"name":"Institute of Dermatology, Guangzhou Dermatology Hospital, Guangzhou Medical University, Guangzhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"232","published-online":{"date-parts":[[2026,5,1]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.7759\/cureus.70697"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1097\/00000372-199808000-00006"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/647684"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202308098"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2017.10.016"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1016\/j.athoracsur.2014.05.026"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1007\/s11307-021-01618-9"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.21037\/tlcr-23-370"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtcvs.2015.05.014"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.21037\/tlcr-22-751"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.21037\/atm-22-6455"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtcvs.2023.02.025"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.7150\/thno.54928"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1038\/s44222-024-00242-w"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1364\/OL.557507"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.4c12485"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1016\/j.pacs.2025.100785"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1016\/j.pacs.2024.100660"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1038\/s41377-025-01992-x"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1117\/1.JMI.5.1.015004"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1117\/1.JBO.24.5.056002"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.498052"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1117\/1.JBO.29.S1.S11518"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1016\/j.injury.2013.03.027"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1016\/j.injury.2020.10.068"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0231488"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aad1278"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.16985"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.425524"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-30471-9"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1142\/S1793545820500236"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aat5857"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1001\/jamadermatol.2018.3269"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-16-3200"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2018.02.002"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1016\/j.pacs.2019.01.004"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1016\/j.pacs.2025.100693"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-53220-y"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1016\/j.jamcollsurg.2017.07.431"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.3390\/ijms17071144"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1148\/radiol.2016160191"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1400643"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.22.12081"},{"key":"B44","first-page":"207","article-title":"Thickness of cadaveric human lung tissue","volume":"29","author":"Chekan","year":"2016","journal-title":"Surg Technol Int"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1117\/1.JMI.5.1.010901"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1042\/BST20180036"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-13175-9"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2022.09.006"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-022-02147-4"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-30265-z"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1906929116"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1038\/s41565-019-0392-3"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1216916110"},{"key":"B54","article-title":"Photothermal nano-vaccine promoting antigen presentation and dendritic cells infiltration for enhanced immunotherapy of melanoma via transdermal microneedles delivery","volume":"2022","author":"Zhu","year":"2022","journal-title":"Research (wash d c)"},{"key":"B55","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-21047-0"},{"key":"B56","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms13193"},{"key":"B57","doi-asserted-by":"publisher","DOI":"10.1093\/bjd\/ljae258"},{"key":"B58","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btw313"},{"key":"B59","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2015.104"},{"key":"B60","doi-asserted-by":"publisher","DOI":"10.1093\/biostatistics\/kxj037"},{"key":"B61","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.2c06629"},{"key":"B62","doi-asserted-by":"publisher","DOI":"10.1038\/laban.1217"},{"key":"B63","doi-asserted-by":"publisher","DOI":"10.3791\/60605"},{"key":"B64","doi-asserted-by":"publisher","DOI":"10.1038\/s41551-020-0527-8"},{"key":"B65","doi-asserted-by":"publisher","DOI":"10.1039\/C6NR08217C"}],"container-title":["Journal of Clinical Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jci.org\/articles\/view\/196095\/files\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T16:34:18Z","timestamp":1779208458000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jci.org\/articles\/view\/196095"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,1]]},"references-count":65,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,3,5]]},"published-print":{"date-parts":[[2026,5,1]]}},"alternative-id":["10.1172\/JCI196095"],"URL":"https:\/\/doi.org\/10.1172\/jci196095","relation":{},"ISSN":["1558-8238"],"issn-type":[{"value":"1558-8238","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,1]]},"article-number":"e196095"}}