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In response to the WHO\u2019s call for therapeutic vaccines to complement antibiotic regimens and reduce tuberculosis (TB) treatment duration, we developed an intranasal DNA vaccine fusing the\n                    <jats:italic>M. tuberculosis<\/jats:italic>\n                    stringent response gene\n                    <jats:italic>relMtb<\/jats:italic>\n                    with the gene encoding the DC-targeting chemokine\n                    <jats:italic>Mip3a<\/jats:italic>\n                    (also known as\n                    <jats:italic>CCL20<\/jats:italic>\n                    ). Administered alongside the first-line regimen, this vaccine accelerated a stable cure in immunocompetent murine TB models, reducing lung inflammation and eliciting robust and sustained RelMtb-stimulated T cell responses systemically and locally. The\n                    <jats:italic>Mip3a\/relMtb<\/jats:italic>\n                    vaccine enhanced DC recruitment, activation, and spatial coordination with T cells, suggesting improved innate-adaptive immune synergy. Notably, it augmented the efficacy of a novel drug-resistant TB regimen as well. Critically, the vaccine induced analogous antigen-stimulated T cell immunity in nonhuman primates, the gold standard for preclinical TB vaccine evaluation, with responses detected in blood and bronchoalveolar lavage mirroring those observed in the murine models. These findings underscore the potential of this strategy to advance therapeutic TB vaccine development targeting\n                    <jats:italic>M. tuberculosis<\/jats:italic>\n                    persisters while providing a framework to define correlates of vaccine-mediated protection.\n                  <\/jats:p>","DOI":"10.1172\/jci196648","type":"journal-article","created":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T17:00:22Z","timestamp":1770138022000},"source":"Crossref","is-referenced-by-count":0,"title":["Immunotherapy targeting drug-tolerant Mycobacterium tuberculosis persisters accelerates tuberculosis cure in preclinical models"],"prefix":"10.1172","volume":"136","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2387-6075","authenticated-orcid":true,"given":"Styliani","family":"Karanika","sequence":"first","affiliation":[{"name":"Center for Tuberculosis Research, Division of Infectious Diseases, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tianyin","family":"Wang","sequence":"additional","affiliation":[{"name":"Center for Tuberculosis Research, Division of Infectious Diseases, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA."},{"name":"W. 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