{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T19:51:47Z","timestamp":1792525907324,"version":"4.5.0"},"reference-count":47,"publisher":"American Society for Clinical Investigation","issue":"8","license":[{"start":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T00:00:00Z","timestamp":1776211200000},"content-version":"vor","delay-in-days":57,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,4,15]]},"abstract":"<jats:p>\n                    Isolating commensal fungi from mouse intestines has been challenging, limiting our understanding of their role in intestinal immune homeostasis and diseases. Using an Fc fusion protein of the C-type lectin receptor Dectin-2, we successfully purified the commensal Ascomycota fungus\n                    <jats:italic>Engyodontium<\/jats:italic>\n                    sp. from mouse feces.\n                    <jats:italic>Engyodontium<\/jats:italic>\n                    enhances the antimicrobial activity of colonic neutrophils via the CARD9 pathway and exacerbates colitis by impairing the colonization of intestinal\n                    <jats:italic>Lactobacillus johnsonii<\/jats:italic>\n                    WXY strain.\n                    <jats:italic>L<\/jats:italic>\n                    .\n                    <jats:italic>johnsonii<\/jats:italic>\n                    produces high levels of l-glutamic acid by expressing the glutaminase-encoding gene\n                    <jats:italic>glsA<\/jats:italic>\n                    to facilitate Treg expansion via enhancing IL-2 receptor signaling. Patients with Crohn disease (CD) and ulcerative colitis harbored increased\n                    <jats:italic>Engyodontium<\/jats:italic>\n                    and decreased\n                    <jats:italic>L<\/jats:italic>\n                    .\n                    <jats:italic>johnsonii<\/jats:italic>\n                    abundance.\n                    <jats:italic>Engyodontium<\/jats:italic>\n                    directly induced calprotectin in human colonic neutrophils, and patients with CD had lower levels of l-glutamic acid, which also promoted human Treg expansion. These findings highlight the\n                    <jats:italic>Engyodontium<\/jats:italic>\n                    -calprotectin axis against the\n                    <jats:italic>Lactobacillus<\/jats:italic>\n                    -glutamate-Treg cascade to aggravate colitis, suggesting commensal\n                    <jats:italic>Engyodontium<\/jats:italic>\n                    -triggered signaling as a therapeutic target for mucosal inflammatory diseases.\n                  <\/jats:p>","DOI":"10.1172\/jci196788","type":"journal-article","created":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T19:40:22Z","timestamp":1772480422000},"source":"Crossref","is-referenced-by-count":1,"title":["Colonic Engyodontium fungus triggers neutrophil antimicrobial activity to suppress Lactobacillus johnsonii\u2013derived glutamic acid\u2013maintained Tregs"],"prefix":"10.1172","volume":"136","author":[{"given":"Xinying","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Gastroenterology and Hepatology, and"},{"name":"Institute of Precision Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong Province, China."},{"name":"College of Basic Medical Science, Dalian Medical University, Dalian, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiyang","family":"Sun","sequence":"additional","affiliation":[{"name":"Institute of Precision Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong Province, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Tan","sequence":"additional","affiliation":[{"name":"Institute of Precision Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong Province, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaoting","family":"Xu","sequence":"additional","affiliation":[{"name":"Institute of Precision Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong Province, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zishan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology, and"},{"name":"Institute of Precision Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong Province, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaile","family":"Ji","sequence":"additional","affiliation":[{"name":"Institute of Precision Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong Province, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ding","family":"Qiu","sequence":"additional","affiliation":[{"name":"Institute of Precision Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong Province, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianping","family":"Deng","sequence":"additional","affiliation":[{"name":"Institute of Precision Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong Province, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bingbing","family":"Feng","sequence":"additional","affiliation":[{"name":"Institute of Precision Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong Province, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueting","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoichiro","family":"Iwakura","sequence":"additional","affiliation":[{"name":"Center for Animal Disease Models, Research Institute for Biomedical Sciences, Tokyo University of Science, Noda, Chiba, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minhu","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chanyan","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ce","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology, and"},{"name":"Institute of Precision Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong Province, China."},{"name":"Animal Experiment Center, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong Province, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"232","published-online":{"date-parts":[[2026,2,17]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1038\/s41575-023-00816-w"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-018-0485-5"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2024.04.043"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1126\/science.1221789"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1126\/science.abd0919"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra0804647"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1038\/nature06005"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)60750-8"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-04502-w"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1126\/science.aao1503"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1093\/gastro\/goz028"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2017.11.017"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1002\/JLB.2RI0119-031R"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwj077"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2010.05.001"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2014.08.005"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.131300"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxr046"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2010.06.001"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0909122107"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1038\/nature12721"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-018-0134-y"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1038\/s42255-022-00558-0"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1007\/s11046-020-00484-3"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2018.11.004"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-019-0144-5"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-22-2145"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.22038\/ijbms.2021.52233.11816"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1016\/j.scib.2024.06.004"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2007.02.009"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.01298"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1038\/nri3228"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-08213-2"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2016.07.015"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1002\/art.10988"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3871"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-30661-5"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07142-4"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.14814\/phy2.14719"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1093\/femsre\/fuaa021"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1038\/s41575-019-0258-z"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-019-0424-4"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2021.612285"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.3390\/ijms13055482"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20061482"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1007\/s11427-020-1703-2"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-05602-3"}],"container-title":["Journal of Clinical Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jci.org\/articles\/view\/196788\/files\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T16:39:05Z","timestamp":1779208745000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jci.org\/articles\/view\/196788"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,17]]},"references-count":47,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,4,15]]},"published-print":{"date-parts":[[2026,4,15]]}},"alternative-id":["10.1172\/JCI196788"],"URL":"https:\/\/doi.org\/10.1172\/jci196788","relation":{},"ISSN":["1558-8238"],"issn-type":[{"value":"1558-8238","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,17]]},"article-number":"e196788"}}