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Understanding these processes is crucial for combating blinding diseases. The visual cycle, operating within PRs and the retinal pigment epithelium (RPE), regenerates 11-\n                    <jats:italic>cis<\/jats:italic>\n                    -retinal to sustain light sensitivity. Retinoids are also present in M\u00fcller glia (MG), hypothesized to supply 11-\n                    <jats:italic>cis<\/jats:italic>\n                    -retinol to cone PRs and retinal ganglion cells (RGCs). To trace retinoid movement through retinal cell types, we used cell-specific knockin of lecithin:retinol acyltransferase (LRAT), which converts retinols into stable retinyl esters (REs). Ectopic LRAT expression in murine PRs, MG, and RGCs resulted in RE synthesis, with REs differing in abundance and isomeric composition across cell types under genetic and light-based perturbations. PR inner segments showed high 11-\n                    <jats:italic>cis<\/jats:italic>\n                    -RE content, suggesting a constant 11-\n                    <jats:italic>cis<\/jats:italic>\n                    -retinoid supply for pigment regeneration. In MG expressing LRAT, all-\n                    <jats:italic>trans<\/jats:italic>\n                    -REs were detected, contrasting with 11-\n                    <jats:italic>cis<\/jats:italic>\n                    -REs in PRs. The MG-specific LRAT phenotype mirrored the RE-rich human neural retina, suggesting human MG may utilize LRAT to maintain retinoid reservoirs. Our findings reveal tightly controlled retinoid flux throughout the mammalian retina that supports sustained vision, expanding understanding of the visual cycle to combat retinal diseases.\n                  <\/jats:p>","DOI":"10.1172\/jci198648","type":"journal-article","created":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T17:00:37Z","timestamp":1763485237000},"source":"Crossref","is-referenced-by-count":3,"title":["Retinol tracing within murine neural retina reveals cell type\u2013specific retinol transport and distribution"],"prefix":"10.1172","volume":"136","author":[{"given":"Zachary J.","family":"Engfer","sequence":"first","affiliation":[{"name":"Brunson Center for Translational Vision Research, Department of Ophthalmology and Visual Sciences, Gavin Herbert Eye Institute, and"},{"name":"Department of Physiology & Biophysics, University of California, Irvine, Irvine, California, 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