{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T01:59:38Z","timestamp":1791597578273,"version":"4.3.4"},"reference-count":70,"publisher":"American Society for Clinical Investigation","issue":"17","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"vor","delay-in-days":49,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the National Key Technology Support Program of the Ministry of Science and Technology of China","award":["2021ZD0203204"],"award-info":[{"award-number":["2021ZD0203204"]}]},{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32571184"],"award-info":[{"award-number":["32571184"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"the National Natural Science Foundation of China Young Student Basic Research Program","award":["324B2030"],"award-info":[{"award-number":["324B2030"]}]},{"name":"the National Key Technology Support Program of the Ministry of Science and Technology of China","award":["2022ZD0206400"],"award-info":[{"award-number":["2022ZD0206400"]}]},{"name":"Beijing Natural Science Foundation","award":["5242008"],"award-info":[{"award-number":["5242008"]}]},{"name":"the Clinical Medicine Plus X-Young Scholars Project of Peking University","award":["PKU2022LCXQ032"],"award-info":[{"award-number":["PKU2022LCXQ032"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,1]]},"abstract":"<jats:p>\n                    Trigeminal neuralgia (TN) is a severe orofacial pain disorder accompanied by anxiety, yet its central mechanisms remain elusive. Analysis of human functional MRI data identified the parafascicular nucleus (PF) as a candidate region. Using a TN mouse model, we uncovered 2 spatially and functionally distinct PF neuronal ensembles that separately encoded sensory and affective dimensions of pain. One population received inhibitory input from GABAergic neurons in the oral spinal trigeminal nucleus (Sp5O) and mediated nociception. The second population, driven by a glutamatergic Sp5O-lateral parabrachial nucleus (lPBN)-PF pathway, encoded pain-related anxiety. The engagement of the anxiety-encoding ensemble lagged behind that of the pain-encoding ensemble, with a shorter delay in females. Single-nucleus RNA sequencing identified\n                    <jats:italic>Col25a1<\/jats:italic>\n                    and\n                    <jats:italic>Syn2<\/jats:italic>\n                    as markers of the anxiety-encoding ensemble. Notably, this population, localized in the medial PF, formed a reciprocal lPBN-mPF-lPBN excitatory feedback loop that sustained affective pain. These findings positioned PF as a key node linking pain and emotion in TN.\n                  <\/jats:p>","DOI":"10.1172\/jci206862","type":"journal-article","created":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T16:01:16Z","timestamp":1784044876000},"source":"Crossref","is-referenced-by-count":1,"title":["Distinct parafascicular neuronal ensembles mediate the sensory and affective dimensions of trigeminal neuralgia"],"prefix":"10.1172","volume":"136","author":[{"given":"Yitian","family":"Lu","sequence":"first","affiliation":[{"name":"Neuroscience Research Institute and Department of Neurobiology, School of Basic Medical Sciences, Key Laboratory for Neuroscience, Ministry of Education\/National Health Commission, Peking University, Beijing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yangyang","family":"Yi","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Cognitive Science and Mental Health, Institute of Psychology, Chinese 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