{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T00:20:47Z","timestamp":1777422047604,"version":"3.51.4"},"reference-count":30,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2009,5,1]],"date-time":"2009-05-01T00:00:00Z","timestamp":1241136000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ann Otol Rhinol Laryngol"],"published-print":{"date-parts":[[2009,5]]},"abstract":"<jats:sec>\n                    <jats:title>Objectives:<\/jats:title>\n                    <jats:p>Chronic otitis media and cholesteatomas cause hearing loss as a result of bony erosion. This bone resorption is known to be more aggressive when cholesteatomas become infected. The most common organism isolated from both diseases is the gram-negative bacterium Pseudomonas aeruginosa. Lipopolysaccharide (LPS), a major virulence factor found in the gram-negative bacterial cell wall, is well known to incite inflammatory bone resorption. The mechanisms underlying this process, however, are poorly understood. In this study, we developed a mouse model of calvarial osteolysis in which resorption was reliably imaged by plain radiography and micro\u2013computed tomography (micro-CT).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>A murine calvarial model was developed to study bone resorption induced by P aeruginosa LPS. Calvariae from wild-type and knockout mice used in this model were imaged by plain radiography and micro-CT.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      A high degree of correlation between plain radiography and micro-CT was identified (R\n                      <jats:sup>2<\/jats:sup>\n                      = 0.8554). Furthermore, maximal LPS-induced bone resorption required functioning toll-like receptor (TLR) 2, TLR4, and myeloid differentiation factor 88 (MyD88).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>We have developed a successful model of inflammatory osteolysis in which plain radiography can reliably delineate induced bone resorption. In vivo, we have shown that P aeruginosa LPS signals via TLR2, as well as TLR4 through MyD88.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/000348940911800512","type":"journal-article","created":{"date-parts":[[2014,10,3]],"date-time":"2014-10-03T14:44:54Z","timestamp":1412347494000},"page":"391-396","source":"Crossref","is-referenced-by-count":9,"title":["Radiographic and Micro\u2014Computed Tomographic Imaging of Lipopolysaccharide-Mediated Bone Resorption"],"prefix":"10.1177","volume":"118","author":[{"given":"Robert","family":"Nason","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong H.","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jae Y.","family":"Jung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard A.","family":"Chole","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2009,5,1]]},"reference":[{"key":"bibr1-000348940911800512","volume-title":"\u00dcber den feineren Bau und die Formen der krankhaften Geschw\u00fclste","author":"M\u00fcller J","year":"1838"},{"key":"bibr2-000348940911800512","volume-title":"Anatomie pathologique du corpus humani","author":"Cruveilhier LJB","year":"1829"},{"key":"bibr3-000348940911800512","doi-asserted-by":"publisher","DOI":"10.1288\/00005537-199211000-00005"},{"key":"bibr4-000348940911800512","doi-asserted-by":"publisher","DOI":"10.1038\/416686a"},{"key":"bibr5-000348940911800512","first-page":"7","volume":"19","author":"Albino AP","year":"1998","journal-title":"Am J Otol"},{"key":"bibr6-000348940911800512","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-199112000-00003"},{"key":"bibr7-000348940911800512","doi-asserted-by":"publisher","DOI":"10.1007\/BF02565215"},{"key":"bibr8-000348940911800512","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2003.10.005"},{"key":"bibr9-000348940911800512","doi-asserted-by":"publisher","DOI":"10.1097\/MLG.0b013e318033783a"},{"key":"bibr10-000348940911800512","author":"Nason R","journal-title":"J Assoc Res Otolaryngol"},{"key":"bibr11-000348940911800512","doi-asserted-by":"publisher","DOI":"10.1038\/nri1391"},{"key":"bibr12-000348940911800512","doi-asserted-by":"publisher","DOI":"10.1126\/science.282.5396.2085"},{"key":"bibr13-000348940911800512","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-06-032961"},{"key":"bibr14-000348940911800512","doi-asserted-by":"publisher","DOI":"10.1038\/ni777"},{"key":"bibr15-000348940911800512","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.45598-0"},{"key":"bibr16-000348940911800512","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.1.348"},{"key":"bibr17-000348940911800512","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80596-8"},{"key":"bibr18-000348940911800512","volume-title":"ImageJ","author":"Rasband WS","year":"1997"},{"key":"bibr19-000348940911800512","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2004.03.003"},{"key":"bibr20-000348940911800512","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2002.17.7.1211"},{"key":"bibr21-000348940911800512","doi-asserted-by":"publisher","DOI":"10.1097\/00129492-200309000-00002"},{"key":"bibr22-000348940911800512","doi-asserted-by":"publisher","DOI":"10.1016\/1043-4666(90)90026-P"},{"key":"bibr23-000348940911800512","doi-asserted-by":"publisher","DOI":"10.1177\/09680519010070030101"},{"key":"bibr24-000348940911800512","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.21.122303.115827"},{"key":"bibr25-000348940911800512","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.170.7.3688"},{"key":"bibr26-000348940911800512","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.169.3.1516"},{"key":"bibr27-000348940911800512","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.4.1736-1742.1999"},{"key":"bibr28-000348940911800512","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M305536200"},{"key":"bibr29-000348940911800512","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.12.6799-6807.2003"},{"key":"bibr30-000348940911800512","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.8.4231-4236.1999"}],"container-title":["Annals of Otology, Rhinology &amp; 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