{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T01:39:57Z","timestamp":1777426797701,"version":"3.51.4"},"reference-count":42,"publisher":"SAGE Publications","issue":"9","license":[{"start":{"date-parts":[[2019,5,1]],"date-time":"2019-05-01T00:00:00Z","timestamp":1556668800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"national natural science foundation of china","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Shanghai Suburb Tertiary Hospital Clinical Capacity Building Project","award":["grant no. SHDC12015905"],"award-info":[{"award-number":["grant no. SHDC12015905"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Ann Otol Rhinol Laryngol"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>Nuocytes are essential in innate type-2 immunity and contribute to the exacerbation of allergic rhinitis (AR). This study aimed to evaluate the intervention of Orai1 on the response of nuocytes from AR mice to interleukin (IL)-33.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      We established a murine model of AR. Nuocytes were obtained from the mouse nasal-associated lymphoid tissue. Then, we assessed expressions of Orai1, Ca\n                      <jats:sup>2+<\/jats:sup>\n                      mean fluorescence intensity (MFI) in nuocytes, and their cellular response to mouse recombinant (rm) IL-33. After that, we administered rmlentivirus vectors (lenti) that encoded small hairpin RNA (shRNA) against ORAI1 (lenti-ORAI1) into nuocytes cultures and again evaluated Orai1 and Ca\n                      <jats:sup>2+<\/jats:sup>\n                      MFI in nuocytes and their response to rmIL-33. Finally, we adoptively transferred nuocytes alone or nuocytes transfected by lenti or lenti-ORAI1 to AR models to investigate their roles during allergic inflammation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      We showed that Orai1 and Ca\n                      <jats:sup>2+<\/jats:sup>\n                      MFI were upregulated in AR mice nuocytes. These cells were induced to produce more IL-5 and IL-13 by rmIL-33. However, the intervention of Orai1 by lenti-ORAI1 in nuocytes decreased Orai1 and Ca\n                      <jats:sup>2+<\/jats:sup>\n                      MFI and reduced productions of aforementioned cytokines even after the administration of rmIL-33. Numbers of sneezing, nasal rubbing, and counts of eosinophils were all enhanced after the adoptive transfer of nuocytes. Concentrations of IL-5, IL-13, and IL-33 in the nasal lavage fluid (NLF) of allergic mice were also increased. However, the adoptive transfer of nuocytes transfected by lenti-ORAI1 decreased aforementioned parameters.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>These findings show that the intervention of Orai1 in nuocytes influences the response of nuocytes to rmIL-33.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/0003489419846142","type":"journal-article","created":{"date-parts":[[2019,5,2]],"date-time":"2019-05-02T02:20:30Z","timestamp":1556763630000},"page":"838-847","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Intervention of Orai1 Influences the Response of Nuocytes From Allergic Rhinitis Mice to IL-33"],"prefix":"10.1177","volume":"128","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3089-4741","authenticated-orcid":false,"given":"Lin","family":"Lin","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology-Head and Neck Surgery, Huashan Hospital of Fudan University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinjin","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology-Head and Neck Surgery, Huashan Hospital of Fudan University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zheng","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology-Head and Neck Surgery, Huashan Hospital of Fudan University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinyue","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology-Head and Neck Surgery, Huashan Hospital of Fudan University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fei","family":"Dai","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology-Head and Neck Surgery, Huashan Hospital of Fudan University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guangbin","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology-Head and Neck Surgery, Huashan Hospital of Fudan University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2019,5,1]]},"reference":[{"key":"bibr1-0003489419846142","doi-asserted-by":"publisher","DOI":"10.1016\/j.iac.2015.12.004"},{"key":"bibr2-0003489419846142","doi-asserted-by":"publisher","DOI":"10.1038\/nri2735"},{"key":"bibr3-0003489419846142","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2016.11.011"},{"key":"bibr4-0003489419846142","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-007-6527-y"},{"key":"bibr5-0003489419846142","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2006.06.014"},{"key":"bibr6-0003489419846142","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2012.08.015"},{"key":"bibr7-0003489419846142","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80625-1"},{"key":"bibr8-0003489419846142","doi-asserted-by":"publisher","DOI":"10.1038\/icb.2010.2"},{"key":"bibr9-0003489419846142","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20052448"},{"key":"bibr10-0003489419846142","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.8.4668"},{"key":"bibr11-0003489419846142","doi-asserted-by":"publisher","DOI":"10.1038\/nature08636"},{"key":"bibr12-0003489419846142","doi-asserted-by":"publisher","DOI":"10.1038\/nature08900"},{"key":"bibr13-0003489419846142","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1003988107"},{"key":"bibr14-0003489419846142","doi-asserted-by":"publisher","DOI":"10.1038\/nature08901"},{"key":"bibr15-0003489419846142","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201107-1258ED"},{"key":"bibr16-0003489419846142","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2104"},{"key":"bibr17-0003489419846142","doi-asserted-by":"publisher","DOI":"10.4193\/Rhin17.065"},{"key":"bibr18-0003489419846142","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceca.2007.03.007"},{"key":"bibr19-0003489419846142","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.179.10.7157"},{"key":"bibr20-0003489419846142","doi-asserted-by":"publisher","DOI":"10.3858\/emm.2012.44.3.013"},{"key":"bibr21-0003489419846142","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201142021"},{"key":"bibr22-0003489419846142","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.181.7.4780"},{"key":"bibr23-0003489419846142","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2005.09.015"},{"key":"bibr24-0003489419846142","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2011.09.041"},{"key":"bibr25-0003489419846142","doi-asserted-by":"publisher","DOI":"10.1111\/cea.12462"},{"key":"bibr26-0003489419846142","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0103540"},{"key":"bibr27-0003489419846142","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1201042109"},{"key":"bibr28-0003489419846142","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.19.1.497"},{"key":"bibr29-0003489419846142","doi-asserted-by":"publisher","DOI":"10.1038\/nri1632"},{"key":"bibr30-0003489419846142","doi-asserted-by":"publisher","DOI":"10.1038\/35037021"},{"key":"bibr31-0003489419846142","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-010-0717-7"},{"key":"bibr32-0003489419846142","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjoto.2010.07.004"},{"key":"bibr33-0003489419846142","doi-asserted-by":"publisher","DOI":"10.1016\/j.anai.2011.09.013"},{"key":"bibr34-0003489419846142","doi-asserted-by":"publisher","DOI":"10.3109\/10520295.2013.839827"},{"key":"bibr35-0003489419846142","doi-asserted-by":"publisher","DOI":"10.1177\/0194599815589582"},{"key":"bibr36-0003489419846142","doi-asserted-by":"publisher","DOI":"10.1159\/000441109"},{"key":"bibr37-0003489419846142","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2014.06.016"},{"key":"bibr38-0003489419846142","doi-asserted-by":"publisher","DOI":"10.1111\/all.12004"},{"key":"bibr39-0003489419846142","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0078099"},{"key":"bibr40-0003489419846142","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2008.03114.x"},{"key":"bibr41-0003489419846142","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2012.02.013"},{"key":"bibr42-0003489419846142","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2011.02735.x"}],"container-title":["Annals 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