{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T01:23:57Z","timestamp":1777425837850,"version":"3.51.4"},"reference-count":48,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2022,6,3]],"date-time":"2022-06-03T00:00:00Z","timestamp":1654214400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Nordmark Pharma GmbH","award":["NM-190418"],"award-info":[{"award-number":["NM-190418"]}]},{"name":"German Federal Ministry of Education and Health","award":["01 EO 0901"],"award-info":[{"award-number":["01 EO 0901"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Ann Otol Rhinol Laryngol"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>Betahistine is frequently used in the pharmacotherapy for Meni\u00e8re\u2019s Disease (MD). Little is known about its mode of action and prescribed dosages vary. While betahistine had an increasing effect on cochlear microcirculation in earlier studies, low dose betahistine of 0.01\u2009mg\/kg bw or less was not able to effect this. Selegiline inhibits monoaminooxidase B and therefore potentially the breakdown of betahistine. The goal of this study was to examine whether the addition of selegiline to low dose betahistine leads to increased cochlear blood flow.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>Twelve Dunkin-Hartley guinea pigs were anesthetized, the cochlea was exposed and a window opened to the stria vascularis. Blood plasma was visualized by injecting fluoresceinisothiocyanate-dextrane and vessel diameter and erythrocyte velocity were evaluated over 20\u2009minutes. One group received low dose betahistine (0.01\u2009mg\/kg bw) and selegiline (1\u2009mg\/kg bw) i.v. while the other group received only selegiline (1\u2009mg\/kg bw) and saline (0.9% NaCl) as placebo i.v.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>Cochlear microcirculation increased significantly ( P\u2009&lt;\u2009.001) in guinea pigs treated with low dose betahistine combined with selegiline by up to 58.3\u2009\u00b1\u200938.7% above baseline over a period of up to 11\u2009minutes. In one guinea pig, the increase was 104.6%. Treatment with Selegiline alone did not affect microcirculation significantly.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>Low dose betahistine increased cochlear microcirculation significantly when combined with selegiline. This should be investigated in further studies regarding dose-effect relation in comparison to betahistine alone. Side effects, in particular regarding circulation, should be considered carefully in view of the clinical applicability of a combination therapy in patients with MD.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/00034894221098803","type":"journal-article","created":{"date-parts":[[2022,6,3]],"date-time":"2022-06-03T04:57:22Z","timestamp":1654232242000},"page":"519-526","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["Low Dose Betahistine in Combination With Selegiline Increases Cochlear Blood Flow in Guinea Pigs"],"prefix":"10.1177","volume":"132","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2486-1756","authenticated-orcid":false,"given":"Benedikt","family":"Kloos","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology, University Hospital, LMU Munich, Munich, Germany"},{"name":"Institute of Surgical Research, Walter-Brendel-Centre of Experimental Medicine, University Hospital, LMU Munich, Munich, Germany"},{"name":"German Center for Vertigo and Dizziness (DSGZ), LMU Munich, Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mattis","family":"Bertlich","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, University Hospital, LMU Munich, Munich, Germany"},{"name":"Department of Dermatology and Allergy, University Hospital, LMU Munich, Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer L.","family":"Spiegel","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, University Hospital, LMU Munich, Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saskia","family":"Freytag","sequence":"additional","affiliation":[{"name":"Molecular Medicine, Harry Perkins Institute of Medical Research, Perth, WA, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5425-7261","authenticated-orcid":false,"given":"Susanne K.","family":"Lauer","sequence":"additional","affiliation":[{"name":"Clinic for Small Animal Surgery and Reproduction, LMU Munich, Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Canis","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, University Hospital, LMU Munich, Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8368-0149","authenticated-orcid":false,"given":"Bernhard G.","family":"Weiss","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, University Hospital, LMU Munich, Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7066-4994","authenticated-orcid":false,"given":"Friedrich","family":"Ihler","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, University Hospital, LMU Munich, Munich, Germany"},{"name":"Institute of Surgical Research, Walter-Brendel-Centre of Experimental Medicine, University Hospital, LMU Munich, Munich, Germany"},{"name":"German Center for Vertigo and Dizziness (DSGZ), LMU Munich, Munich, Germany"},{"name":"Department of Ear, Nose and Throat Diseases, Head and Neck Surgery, Greifswald, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2022,6,3]]},"reference":[{"key":"bibr1-00034894221098803","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2016.28"},{"key":"bibr2-00034894221098803","doi-asserted-by":"publisher","DOI":"10.1159\/000475875"},{"key":"bibr3-00034894221098803","unstructured":"Ferrara S, Dispenza F. Cause, pathogenesis, clinical manifestations and treatment of meniere\u2019s disease and endolymphatic hydrops. In: Dispenza MF (ed.) Sensorineural Hearing Loss: Pathophysiology, Diagnosis and Treatment. Nova Science Publishers, Inc; 2019; 217\u2013231."},{"key":"bibr4-00034894221098803","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2016.00182"},{"key":"bibr5-00034894221098803","doi-asserted-by":"publisher","DOI":"10.1055\/s-0039-3402065"},{"key":"bibr6-00034894221098803","doi-asserted-by":"publisher","DOI":"10.1016\/j.heares.2012.12.004"},{"key":"bibr7-00034894221098803","doi-asserted-by":"publisher","DOI":"10.1016\/j.otc.2010.05.007"},{"key":"bibr8-00034894221098803","doi-asserted-by":"publisher","DOI":"10.1177\/000348949009900611"},{"key":"bibr9-00034894221098803","doi-asserted-by":"publisher","DOI":"10.1097\/MAO.0000000000002502"},{"key":"bibr10-00034894221098803","doi-asserted-by":"publisher","DOI":"10.1017\/S0022215114001972"},{"key":"bibr11-00034894221098803","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjorl.2015.08.021"},{"key":"bibr12-00034894221098803","first-page":"CD008234","volume":"3","author":"Pullens B","year":"2011","journal-title":"Cochrane Database Syst Rev"},{"key":"bibr13-00034894221098803","doi-asserted-by":"publisher","DOI":"10.2165\/00023210-200115110-00004"},{"key":"bibr14-00034894221098803","doi-asserted-by":"publisher","DOI":"10.1159\/000368293"},{"key":"bibr15-00034894221098803","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0039086"},{"key":"bibr16-00034894221098803","doi-asserted-by":"publisher","DOI":"10.3109\/14992027.2014.917208"},{"key":"bibr17-00034894221098803","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2017.08.015"},{"key":"bibr18-00034894221098803","doi-asserted-by":"publisher","DOI":"10.2165\/00002018-200629110-00004"},{"key":"bibr19-00034894221098803","volume":"1","author":"James AL","year":"2001","journal-title":"Cochrane Database Syst Rev"},{"key":"bibr20-00034894221098803","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.h6816"},{"key":"bibr21-00034894221098803","doi-asserted-by":"publisher","DOI":"10.1007\/s00405-013-2596-8"},{"key":"bibr22-00034894221098803","doi-asserted-by":"publisher","DOI":"10.1177\/0091270010369241"},{"key":"bibr23-00034894221098803","doi-asserted-by":"publisher","DOI":"10.1007\/s00415-018-8809-8"},{"key":"bibr24-00034894221098803","first-page":"65","volume-title":"International Review of Neurobiology","author":"Magyar K","year":"2011"},{"key":"bibr25-00034894221098803","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.63.7_suppl_2.S32"},{"key":"bibr26-00034894221098803","doi-asserted-by":"publisher","DOI":"10.1016\/j.npep.2019.101942"},{"key":"bibr27-00034894221098803","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2018.10.032"},{"key":"bibr28-00034894221098803","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2018.00431"},{"key":"bibr29-00034894221098803","doi-asserted-by":"publisher","DOI":"10.3109\/14992027.2013.790564"},{"key":"bibr30-00034894221098803","doi-asserted-by":"publisher","DOI":"10.1097\/MAO.0000000000001523"},{"key":"bibr31-00034894221098803","doi-asserted-by":"publisher","DOI":"10.1177\/000348941312200711"},{"key":"bibr32-00034894221098803","doi-asserted-by":"publisher","DOI":"10.1007\/s00405-009-1036-2"},{"key":"bibr33-00034894221098803","unstructured":"Russel W, Burch L. The Principles of Humane Experimental Technique, special ed. Universities Federation for Animal Welfare; 1992."},{"key":"bibr34-00034894221098803","doi-asserted-by":"publisher","DOI":"10.1515\/bmte.1997.42.6.168"},{"key":"bibr35-00034894221098803","doi-asserted-by":"publisher","DOI":"10.1016\/0026-2862(74)90043-0"},{"key":"bibr36-00034894221098803","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-009-0754-z"},{"key":"bibr37-00034894221098803","doi-asserted-by":"publisher","DOI":"10.1016\/j.mehy.2013.10.015"},{"key":"bibr38-00034894221098803","doi-asserted-by":"publisher","DOI":"10.1016\/s0378-5955(00)00005-8"},{"key":"bibr39-00034894221098803","doi-asserted-by":"publisher","DOI":"10.3949\/ccjm.77a.09103"},{"key":"bibr40-00034894221098803","doi-asserted-by":"publisher","DOI":"10.1515\/BMC.2011.030"},{"key":"bibr41-00034894221098803","doi-asserted-by":"publisher","DOI":"10.1002\/cmdc.201500408"},{"issue":"1","key":"bibr42-00034894221098803","first-page":"41","volume":"8","author":"Abdanipour A","year":"2018","journal-title":"Biomed Rep"},{"key":"bibr43-00034894221098803","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2017.01.005"},{"key":"bibr44-00034894221098803","doi-asserted-by":"publisher","DOI":"10.3109\/00016489709124019"},{"key":"bibr45-00034894221098803","doi-asserted-by":"publisher","DOI":"10.3109\/00016489.2015.1072873"},{"issue":"13","key":"bibr46-00034894221098803","first-page":"1879","volume":"18","author":"Marconi S","year":"2014","journal-title":"Eur Rev Med Pharmacol Sci"},{"key":"bibr47-00034894221098803","doi-asserted-by":"publisher","DOI":"10.1007\/s00415-017-8585-x"},{"key":"bibr48-00034894221098803","doi-asserted-by":"publisher","DOI":"10.1007\/s00702-012-0927-3"}],"container-title":["Annals of Otology, Rhinology &amp; Laryngology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/00034894221098803","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/00034894221098803","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/00034894221098803","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T11:30:11Z","timestamp":1777375811000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.sagepub.com\/doi\/10.1177\/00034894221098803"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,3]]},"references-count":48,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2023,5]]}},"alternative-id":["10.1177\/00034894221098803"],"URL":"https:\/\/doi.org\/10.1177\/00034894221098803","relation":{},"ISSN":["0003-4894","1943-572X"],"issn-type":[{"value":"0003-4894","type":"print"},{"value":"1943-572X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,3]]}}}