{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T10:02:49Z","timestamp":1785232969167,"version":"3.55.0"},"reference-count":46,"publisher":"SAGE Publications","issue":"9","license":[{"start":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T00:00:00Z","timestamp":1776902400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["R01HL157039"],"award-info":[{"award-number":["R01HL157039"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Ohio State College of Medicine"},{"name":"Carolyn L. Kuckein Student Research Fellowship"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Ann Otol Rhinol Laryngol"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>Airway reconstruction triggers a surgery-induced stress (SIS) response, but its effect on cartilage regeneration remains elusive. SOX9 regulates cartilage regeneration by promoting type II collagen production and may aid in repair; however, the role of SOX9 in response to SIS is unclear. We utilized a mouse model to validate SOX9 nuclear expression in tracheal chondrocytes and assess its localization after segmental tracheal replacement.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>Tracheas from C57BL\/6J mice were digested using Dispase-Collagenase-Trypsin, then collagenase II to isolate chondrocytes for primary culture. Syngeneic tracheal grafts were harvested from donor animals, and a 3 to 4\u2009mm tracheal graft was implanted orthotopically to assess chondrocyte function in vivo. At 1\u2009month, graft, anastomosis, and adjacent host tissues were harvested. Samples were stained for SOX9 and COL2A1, and their localization was analyzed with ImageJ.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      Primary tracheal chondrocytes showed strong nuclear SOX9 and cytoplasmic COL2A1 expression. One\u2009month after orthotopic tracheal replacement, SOX9 nuclear colocalization increased in the host and anastomosis regions (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      \u2009=\u2009.0269 and\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      \u2009=\u2009.0262, respectively), but not in the graft (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      \u2009=\u2009.5958). COL2A1 intensity in the cartilage matrix was significantly higher in graft, host, and anastomosis compared to native cartilage (all\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      \u2009&lt;\u2009.01), with no morphological differences.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>After tracheal reconstruction, increased SOX9 and COL2A1 intensities indicate a potential chondrocyte response to SIS. SOX9 nuclear localization may be involved in regulating COL2A1 in tracheal chondrocytes during cartilage injury and repair. This supports a possible role for SOX9 in maintaining cartilage function and its potential as a therapeutic target to improve graft integration and airway stability.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/00034894261440648","type":"journal-article","created":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T04:19:29Z","timestamp":1777004369000},"page":"698-706","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Surgery-Induced Stress Promotes SOX9 Nuclear Localization and Increases COL2A1 in Tracheal Cartilage"],"prefix":"10.1177","volume":"135","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0191-0941","authenticated-orcid":false,"given":"Sovannarath","family":"Pong","sequence":"first","affiliation":[{"name":"The Ohio State University College of Medicine, Columbus, OH, USA"},{"name":"Center of Regenerative Medicine, Abigail Wexner Research Institute, Nationwide Children\u2019s Hospital, Columbus, OH, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9849-6881","authenticated-orcid":false,"given":"Lumei","family":"Liu","sequence":"additional","affiliation":[{"name":"Center of Regenerative Medicine, Abigail Wexner Research Institute, Nationwide Children\u2019s Hospital, Columbus, OH, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jazmin","family":"Calyeca","sequence":"additional","affiliation":[{"name":"Center of Regenerative Medicine, Abigail Wexner Research Institute, Nationwide Children\u2019s Hospital, Columbus, OH, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1238-2953","authenticated-orcid":false,"given":"Ada C.","family":"Sher","sequence":"additional","affiliation":[{"name":"The Ohio State University College of Medicine, Columbus, OH, USA"},{"name":"Center of Regenerative Medicine, Abigail Wexner Research Institute, Nationwide Children\u2019s Hospital, Columbus, OH, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Sussman","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology-Head &amp; Neck Surgery, Ohio State University Wexner Medical Center, Columbus, OH, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tendy","family":"Chiang","sequence":"additional","affiliation":[{"name":"Center of Regenerative Medicine, Abigail Wexner Research Institute, Nationwide Children\u2019s Hospital, Columbus, OH, USA"},{"name":"Department of Otolaryngology-Head &amp; Neck Surgery, Ohio State University Wexner Medical Center, Columbus, OH, USA"},{"name":"Department of Otolaryngology-Head &amp; Neck Surgery, Nationwide Children\u2019s Hospital, Columbus, OH, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,4,23]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1177\/0148607113496117"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.186263"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/lary.30206"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.3390\/bioengineering8010013"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0127166"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.thorsurg.2013.09.003"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.128.5.3391"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1001\/jamaoto.2018.3276"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1177\/20417314211017417"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2012.10.004"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2019.11.008"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41536-023-00312-4"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.3390\/biom11060866"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms221910322"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-2277.2005.00082.x"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/ejcts\/ezx317"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng0697-174"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.664168"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.130.6.732"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2020.04.008"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.22192"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2020.585640"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/humu.23888"},{"key":"e_1_3_3_25_2","author":"Unger S","unstructured":"Unger S, Scherer G, Superti-Furga A. 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