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Discriminant analyses of the maximum concentration of 13 elements measured at several sites within each of 32 stapes differentiated otosclerotic from unafflicted individuals well above the 95% confidence level. Eight of the 9 control (unafflicted) and 21 of the 23 afflicted stapes were correctly classified. In descending order of contribution to the discriminant function, the elements are Zn &gt; Cr &gt; K &gt; Ca &gt; Si &gt; Mn &gt; Na &gt; Al &gt; Mg &gt; P &gt; Fe &gt; S &gt; Ti. Zinc and chromium account for much of the difference, but discriminant analyses excluding them still distinguish the two groups at the 95% confidence level. These results are consistent with previous reports of high levels of alkaline phosphatase, a zinc-containing enzyme, in afflicted stapes. But the broad spectrum of elements capable of distinguishing otosclerotic stapes warrants study of additional zinc-containing and other metal-containing or metal-activated moieties.<\/jats:p>","DOI":"10.1177\/000348949310200506","type":"journal-article","created":{"date-parts":[[2014,10,3]],"date-time":"2014-10-03T14:38:05Z","timestamp":1412347085000},"page":"353-358","source":"Crossref","is-referenced-by-count":0,"title":["Composition of the Otosclerotic Stapes: Electron Microprobe Analyses"],"prefix":"10.1177","volume":"102","author":[{"given":"Gary D.","family":"Rosenberg","sequence":"first","affiliation":[{"name":"Indianapolis, Indiana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laverne B.","family":"Tubergen","sequence":"additional","affiliation":[{"name":"Indianapolis, 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