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S-35 immunoreactivity was observed clearly in olfactory receptor neurons (ORNs) and olfactory nerve bundles that were identified previously with antibodies to olfactory marker protein (OMP) and neuron-specific enolase (NSE). Throughout all ages, the mean number of ORNs immunoreactive for OMP did not change significantly, whereas the mean number of NSE- and S-35-immunoreactive ORNs declined markedly in the postnatal infant, young, and old patients when compared with that of the prenatal fetuses. S-35-immunoreactive ORNs decreased significantly in AD patients when compared with AD control patients. These results indicate that ORNs in humans express S-35 and that there is an age-related trend in the expression of S-35. Furthermore, the marked decrease of S-35 expression in ORNs of AD patients suggests that cell excitability associated with calcium ions and cell protective function against overload of intracellular calcium ions decline in these patients.<\/jats:p>","DOI":"10.1177\/000348949610500208","type":"journal-article","created":{"date-parts":[[2014,10,3]],"date-time":"2014-10-03T14:38:05Z","timestamp":1412347085000},"page":"132-139","source":"Crossref","is-referenced-by-count":15,"title":["Ontogenetic Expression of Spot 35 Protein (Calbindin-D28K) in Human Olfactory Receptor Neurons and its Decrease in Alzheimer's Disease Patients"],"prefix":"10.1177","volume":"105","author":[{"given":"Masuo","family":"Yamagishi","sequence":"first","affiliation":[{"name":"Ina, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigeru","family":"Takami","sequence":"additional","affiliation":[{"name":"Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas V.","family":"Getchell","sequence":"additional","affiliation":[{"name":"Lexington, Kentucky"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[1996,2,1]]},"reference":[{"key":"bibr1-000348949610500208","doi-asserted-by":"publisher","DOI":"10.1016\/0166-2236(89)90089-1"},{"key":"bibr2-000348949610500208","doi-asserted-by":"publisher","DOI":"10.1016\/0166-2236(92)90081-I"},{"key":"bibr3-000348949610500208","doi-asserted-by":"publisher","DOI":"10.1016\/0968-0004(91)90041-S"},{"key":"bibr4-000348949610500208","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(91)90120-O"},{"key":"bibr5-000348949610500208","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(89)90631-9"},{"key":"bibr6-000348949610500208","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902800203"},{"key":"bibr7-000348949610500208","doi-asserted-by":"publisher","DOI":"10.1016\/0166-2236(92)90067-I"},{"key":"bibr8-000348949610500208","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(87)90974-7"},{"key":"bibr9-000348949610500208","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(89)90793-2"},{"key":"bibr10-000348949610500208","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(90)90487-T"},{"key":"bibr11-000348949610500208","doi-asserted-by":"publisher","DOI":"10.3109\/00016489209137485"},{"key":"bibr12-000348949610500208","doi-asserted-by":"publisher","DOI":"10.1007\/BF00964462"},{"key":"bibr13-000348949610500208","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(84)90232-5"},{"key":"bibr14-000348949610500208","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(85)90455-8"},{"key":"bibr15-000348949610500208","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(85)80888-7"},{"key":"bibr16-000348949610500208","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/14.16.6768"},{"key":"bibr17-000348949610500208","doi-asserted-by":"publisher","DOI":"10.1210\/mend-2-5-465"},{"key":"bibr18-000348949610500208","doi-asserted-by":"publisher","DOI":"10.1089\/dna.1988.7.585"},{"key":"bibr19-000348949610500208","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(93)91417-Q"},{"key":"bibr20-000348949610500208","doi-asserted-by":"crossref","unstructured":"Yamagishi M, Ishizuka Y, Fujiwara M, Nakamura H, Igarashi S, Kuwano R. 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