{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T00:08:39Z","timestamp":1777421319246,"version":"3.51.4"},"reference-count":19,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[1997,8,1]],"date-time":"1997-08-01T00:00:00Z","timestamp":870393600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ann Otol Rhinol Laryngol"],"published-print":{"date-parts":[[1997,8]]},"abstract":"<jats:p>Immunohistochemical analyses were used to investigate the distribution of lymphocytes and macrophages in routine human temporal bone sections obtained from a subject with acute suppurative otitis media. Primary antibodies specific for human CD3 and CD43 (T-lymphocytes), CD20 (B-lymphocytes), CD45 (leukocyte common antigen), and CD68 (macrophages) were used. As a pretreatment, the sections were soaked in antigen retrieval solution (saturated sodium hydroxide-methanol solution in methanol at a ratio of 1:3). A second antigen retrieval procedure (microwave treatment in 1 % zinc sulfate) was also employed for identifying CD3-positive cells. Then the avidin-biotin-peroxidase complex technique was performed. Positive reactions to all antibodies but anti-CD68 were observed in the mucosa of the eustachian tube, tympanic cavity, and mastoid air cells. Particularly, cells positive to anti-CD3 or anti-CD43 were making a diffuse invasion upon the lamina propria. CD68-positive cells were scattered only in the effusion of mastoid air cells. These results suggest that the retrospective immunohistochemical study of archival temporal bone sections is a promising approach to investigate the pathogenesis of otitis media.<\/jats:p>","DOI":"10.1177\/000348949710600809","type":"journal-article","created":{"date-parts":[[2014,10,3]],"date-time":"2014-10-03T14:38:05Z","timestamp":1412347085000},"page":"662-668","source":"Crossref","is-referenced-by-count":12,"title":["Immunohistochemistry of Lymphocytes and Macrophages in Human Celloidin-Embedded Temporal Bone Sections with Acute Otitis Media"],"prefix":"10.1177","volume":"106","author":[{"given":"Tetsuya","family":"Ganbo","sequence":"first","affiliation":[{"name":"Pittsburgh, Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isamu","family":"Sando","sequence":"additional","affiliation":[{"name":"Pittsburgh, Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiaki","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Pittsburgh, Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carey D.","family":"Balaban","sequence":"additional","affiliation":[{"name":"Pittsburgh, Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masaharu","family":"Sudo","sequence":"additional","affiliation":[{"name":"Pittsburgh, Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1997,8,1]]},"reference":[{"key":"bibr1-000348949710600809","doi-asserted-by":"publisher","DOI":"10.1177\/00034894800890S336"},{"key":"bibr2-000348949710600809","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.1981.00790450004003"},{"key":"bibr3-000348949710600809","doi-asserted-by":"publisher","DOI":"10.1177\/000348947108000611"},{"key":"bibr4-000348949710600809","doi-asserted-by":"publisher","DOI":"10.3109\/00016488409132917"},{"key":"bibr5-000348949710600809","doi-asserted-by":"publisher","DOI":"10.1177\/40.6.1588025"},{"key":"bibr6-000348949710600809","doi-asserted-by":"publisher","DOI":"10.1177\/000348948609500311"},{"key":"bibr7-000348949710600809","doi-asserted-by":"publisher","DOI":"10.1177\/39.6.1709656"},{"key":"bibr8-000348949710600809","doi-asserted-by":"publisher","DOI":"10.1007\/BF01005033"},{"key":"bibr9-000348949710600809","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.42.11.1194"},{"key":"bibr10-000348949710600809","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830171023"},{"key":"bibr11-000348949710600809","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.42.9.953"},{"key":"bibr12-000348949710600809","first-page":"183","volume":"58","author":"Ishii Y","year":"1984","journal-title":"Clin Exp Immunol"},{"key":"bibr13-000348949710600809","first-page":"415","volume":"129","author":"Cartun RW","year":"1987","journal-title":"Am J Pathol"},{"key":"bibr14-000348949710600809","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830101003"},{"key":"bibr15-000348949710600809","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711490405"},{"key":"bibr16-000348949710600809","first-page":"1359","volume":"142","author":"Falini B","year":"1993","journal-title":"Am J Pathol"},{"key":"bibr17-000348949710600809","doi-asserted-by":"publisher","DOI":"10.3109\/00016488409132919"},{"key":"bibr18-000348949710600809","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-197807000-00009"},{"key":"bibr19-000348949710600809","doi-asserted-by":"publisher","DOI":"10.1288\/00005537-195508000-00003"}],"container-title":["Annals of Otology, Rhinology &amp; Laryngology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/000348949710600809","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/000348949710600809","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T11:16:25Z","timestamp":1777374985000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/000348949710600809"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,8]]},"references-count":19,"journal-issue":{"issue":"8","published-print":{"date-parts":[[1997,8]]}},"alternative-id":["10.1177\/000348949710600809"],"URL":"https:\/\/doi.org\/10.1177\/000348949710600809","relation":{},"ISSN":["0003-4894","1943-572X"],"issn-type":[{"value":"0003-4894","type":"print"},{"value":"1943-572X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,8]]}}}