{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T06:06:41Z","timestamp":1777442801794,"version":"3.51.4"},"reference-count":27,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2021,7,9]],"date-time":"2021-07-09T00:00:00Z","timestamp":1625788800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Appl Spectrosc"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:p>The global fight against mosquito-borne viral diseases has in recent years been bolstered by the introduction of the endosymbiotic bacteria Wolbachia to vector populations, which in host mosquitoes suppresses the transmissibility of several viruses. Researchers engaged on this front of the battle often need to know the Wolbachia infection status of individual mosquitoes, as the intervention progresses and the mosquitoes become established in the target population. Previously, we successfully applied attenuated total reflection Fourier transform infrared spectroscopy to the detection of Wolbachia in adult Aedes aegypti mosquitoes; here we apply the same principles to Aedes eggs, with sensitivity and selectivity\u2009&gt;\u20090.95. Further, we successfully distinguish between infections in eggs of the wMel and wMelPop strains of Wolbachia pipientis, with a classification error of 3% . The disruption of host lipid profile by Wolbachia is found to be a key driver in spectral differences between these sample classes.<\/jats:p>","DOI":"10.1177\/00037028211027140","type":"journal-article","created":{"date-parts":[[2021,6,10]],"date-time":"2021-06-10T23:23:21Z","timestamp":1623367401000},"page":"1003-1011","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Detection and Identification of\n                    <i>Wolbachia pipientis<\/i>\n                    Strains in Mosquito Eggs Using Attenuated Total Reflection Fourier Transform Infrared (ATR FT-IR) Spectroscopy"],"prefix":"10.1177","volume":"75","author":[{"given":"Dale","family":"Christensen","sequence":"first","affiliation":[{"name":"Centre for Biospectroscopy, School of Chemistry, Monash University, Clayton, Australia"},{"name":"ANSTO Australian Synchrotron, Clayton, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aazam","family":"Khoshmanesh","sequence":"additional","affiliation":[{"name":"Centre for Biospectroscopy, School of Chemistry, Monash University, Clayton, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Perez-Guaita","sequence":"additional","affiliation":[{"name":"Centre for Biospectroscopy, School of Chemistry, Monash University, Clayton, Australia"},{"name":"Department of Analytical Chemistry, University of Valencia, Burjassot, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Inaki","family":"Iturbe-Ormaetxe","sequence":"additional","affiliation":[{"name":"Institute of Vector-Borne Diseases, Monash University, Clayton, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4131-3615","authenticated-orcid":false,"given":"Scott","family":"O\u2019Neill","sequence":"additional","affiliation":[{"name":"World Mosquito Program, Ho Chi Minh City, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3581-447X","authenticated-orcid":false,"given":"Bayden R.","family":"Wood","sequence":"additional","affiliation":[{"name":"Centre for Biospectroscopy, School of Chemistry, Monash University, Clayton, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2021,7,9]]},"reference":[{"key":"bibr1-00037028211027140","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001760"},{"key":"bibr2-00037028211027140","doi-asserted-by":"publisher","DOI":"10.1038\/nature12060"},{"key":"bibr3-00037028211027140","unstructured":"World Health Organization (WHO). \u201cDengue and Severe Dengue\u201d. 2020. https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/dengue-and-severe-dengue [accessed Apr 15 2021]."},{"issue":"3","key":"bibr4-00037028211027140","first-page":"329","volume":"44","author":"Hertig M.","year":"1924","journal-title":"J. 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