{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T07:57:25Z","timestamp":1790582245662,"version":"4.1.0"},"reference-count":45,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2024,12,16]],"date-time":"2024-12-16T00:00:00Z","timestamp":1734307200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100003093","name":"Ministry of Higher Education Malaysia","doi-asserted-by":"crossref","award":["Fundamental Research Grant Scheme (FRGS\/1\/2021\/STG"],"award-info":[{"award-number":["Fundamental Research Grant Scheme (FRGS\/1\/2021\/STG"]}],"id":[{"id":"10.13039\/501100003093","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003093","name":"Ministry of Higher Education Malaysia","doi-asserted-by":"crossref","award":["Fundamental Research Grant Scheme for Research Acc"],"award-info":[{"award-number":["Fundamental Research Grant Scheme for Research Acc"]}],"id":[{"id":"10.13039\/501100003093","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Appl Spectrosc"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:p>\n                    Leptospirosis is an acute bacterial febrile disease affecting humans and animals in many tropical and subtropical countries. This work presents an optimization of surface-enhanced Raman spectroscopy (SERS) substrates to probe vibrational spectroscopic detail from\n                    <jats:italic>Leptospira<\/jats:italic>\n                    deoxyribonucleic acid (DNA). The pathogenic gene of LipL32 was used as a biomarker. The SERS substrates were based on a photonic crystal (PC) structure embedded with bi-metallic gold and silver nanoparticles (PC@AuAg NPs). The localized plasmonic resonance of AuAg NPs was coupled to the Raman modes of the target through SERS interaction. Prior to detection, the AuAg NPs were functionalized with chemical linkers to facilitate specific conjugation between metallic surfaces and DNA biomolecules. The immobilization and hybridization of probe DNA to their complementary target DNA (cDNA) created duplex formation for detection. The configuration was also tested with non-complementary DNA to verify detection specificity. Prominent SERS peaks were recorded, and the characteristic intensity decreased after cDNA hybridization due to less base interaction after complementary pairing. Distinct SERS behavior from the negative control test was also observed in non-complementary interaction. The configuration is highly attractive and can be potentially extended for sensitive and label-free detection of leptospiral DNA, paving the way for alternative diagnosis of leptospirosis.\n                  <\/jats:p>","DOI":"10.1177\/00037028241303780","type":"journal-article","created":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T02:05:03Z","timestamp":1734401103000},"page":"986-996","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Surface-Enhanced Raman Spectroscopy (SERS) Substrates Based on Photonic Crystal Embedded Bi-Metallic Nanoparticles for Leptospiral DNA Detection"],"prefix":"10.1177","volume":"79","author":[{"given":"Anis Athirah","family":"Abdul Razak","sequence":"first","affiliation":[{"name":"Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, Kuala Nerus, Kuala Terengganu, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liyana","family":"Shatar","sequence":"additional","affiliation":[{"name":"Physics Department, Faculty of Science, Universiti Teknologi Malaysia, Johor Bahru, Skudai, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aima","family":"Ramli","sequence":"additional","affiliation":[{"name":"Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, Kuala Nerus, Kuala Terengganu, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Syara","family":"Kassim","sequence":"additional","affiliation":[{"name":"Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, Kuala Nerus, Kuala Terengganu, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohd Sabri","family":"Mohd Ghazali","sequence":"additional","affiliation":[{"name":"Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, Kuala Nerus, Kuala Terengganu, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui Yee","family":"Chee","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia"},{"name":"Wireless and Photonic Networks Research Centre, Faculty of Engineering, UPM, Serdang, Selangor, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rozalina","family":"Zakaria","sequence":"additional","affiliation":[{"name":"Photonic Research Centre, Faculty of Science, Universiti Malaya, Kuala Lumpur, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohd Adzir","family":"Mahdi","sequence":"additional","affiliation":[{"name":"Wireless and Photonic Networks Research Centre, Faculty of Engineering, UPM, Serdang, Selangor, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3099-6390","authenticated-orcid":false,"given":"Fariza Hanim","family":"Suhailin","sequence":"additional","affiliation":[{"name":"Physics Department, Faculty of Science, Universiti Teknologi Malaysia, Johor Bahru, Skudai, Malaysia"},{"name":"Laser Center, Ibnu Sina Institute for Scientific &amp; Industrial Research (ISI-SIR), UTM, Johor Bahru, Johor, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2024,12,16]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-019-03797-4"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1590\/s1678-9946201860023"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-64172-4"},{"key":"e_1_3_3_5_2","first-page":"65","article-title":"Leptospirosis in Humans","volume":"387","author":"Haake D.A.","year":"2015","unstructured":"Haake D.A., Levett P.N.. \u201cLeptospirosis in Humans\u201d. 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