{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T11:25:40Z","timestamp":1788866740879,"version":"build-2803163510"},"reference-count":26,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2013,7,11]],"date-time":"2013-07-11T00:00:00Z","timestamp":1373500800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Ann Clin Biochem"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Urinary 18-hydroxycortisol has been investigated as a marker of aldosterone-producing adenoma (APA). The aim of this study was to develop and validate a method for the measurement of 18-hydroxycortisol using liquid chromatography-tandem mass spectrometry (LC-MS\/MS).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Urine was collected over a 24-hour period in patients with APA ( n\u2009=\u200911), idiopathic hyperaldosteronism (IHA, n\u2009=\u20099), and essential hypertension (EH, n\u2009=\u20096). 18-Hydroxycortisol was extracted in solid-phase, and measured by LC-MS\/MS based on selected reaction monitoring.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The method allowed quantification of 18-hydroxycortisol with a lower quantification limit of 0.26\u2009nmol\/L, intra- and inter-assay coefficients of variation of &lt;3.4% and a range of analytical recovery of 98.0\u2013103.7%. Urinary 18-hydroxycortisol excretion for APA, IHA and EH were determined as 725 (SD 451), 102 (SD 68) and 88 (SD 76) nmol\/day, respectively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The proposed method met the basic analytical requirements and was considered to be useful in the screening and differential diagnosis of APA.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/0004563213476272","type":"journal-article","created":{"date-parts":[[2013,7,12]],"date-time":"2013-07-12T00:24:45Z","timestamp":1373588685000},"page":"450-456","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":9,"title":["Quantification of urinary 18-hydroxycortisol using LC-MS\/MS"],"prefix":"10.1177","volume":"50","author":[{"given":"Shigeki","family":"Jin","sequence":"first","affiliation":[{"name":"Faculty of Health Sciences, Hokkaido University, Sapporo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norio","family":"Wada","sequence":"additional","affiliation":[{"name":"Department of Diabetes and Endocrinology, Sapporo City General Hospital, Sapporo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuji","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Faculty of Health Sciences, Hokkaido University, Sapporo, Japan"},{"name":"Department of Clinical Laboratory, Sapporo City General Hospital, Sapporo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu-Ping","family":"Hui","sequence":"additional","affiliation":[{"name":"Faculty of Health Sciences, Hokkaido University, Sapporo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshihiro","family":"Sakurai","sequence":"additional","affiliation":[{"name":"Faculty of Health Sciences, Hokkaido University, Sapporo, 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