{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T02:08:33Z","timestamp":1791598113902,"version":"4.3.4"},"reference-count":21,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2013,7,11]],"date-time":"2013-07-11T00:00:00Z","timestamp":1373500800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Ann Clin Biochem"],"published-print":{"date-parts":[[2014,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Glomerular filtration rate (GFR) and mortality is more accurately determined by gold standard measures than serum creatinine-based estimates in cirrhosis. No formal validation of any gold standard method has been reported.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      An isocratic methanol-based method incorporating the reference standard iohexol-related compound C was developed and validated in 12 patients with cirrhosis by simultaneously determining GFR using iohexol and chromium-51 labelled ethylenediamine tetraacetic acid (\n                      <jats:sup>51<\/jats:sup>\n                      Cr-EDTA) clearance. Iohexol pharmacokinetics was also studied with the collection of blood and ascitic fluid at intervals following an iohexol bolus.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Triplicate assays produced a linear calibration curve ( R\n                      <jats:sup>2\u2009<\/jats:sup>\n                      =\u20090.99, N\u2009=\u20095) over an iohexol concentration range of 23.6\u2013755\u2009\u00b5g\/L. Mean (range) extraction recovery of iohexol from serum was greater than 95% (94\u201397%), with an intra-day coefficient of variation less than 3%. Twelve patients with cirrhosis with mean Child-Pugh score of 9 displayed a mean difference (bias) \u22121.3\u2009mL\/min\/1.73\u2009m\n                      <jats:sup>2<\/jats:sup>\n                      (\u221218 to\u2009+\u200916) comparing iohexol with\n                      <jats:sup>51<\/jats:sup>\n                      Cr-EDTA. Iohexol equilibrated between blood and ascitic compartments after 4\u2009h.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      A simple, cheap, and accurate isocratic, methanol-based method for the determination of iohexol concentrations is described, validated according to Food and Drug Administration guidance. Iohexol demonstrated comparable performance with\n                      <jats:sup>51<\/jats:sup>\n                      Cr-EDTA in determining GFR. Delayed equilibrium of iohexol between blood and ascitic compartments suggests sampling beyond 4\u2009h would improve accuracy of GFR determinations in patients with cirrhosis.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/0004563213487715","type":"journal-article","created":{"date-parts":[[2013,7,12]],"date-time":"2013-07-12T00:24:45Z","timestamp":1373588685000},"page":"80-88","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":11,"title":["Application of an isocratic methanol-based HPLC method for the determination of iohexol concentrations and glomerular filtration rate in patients with cirrhosis"],"prefix":"10.1177","volume":"51","author":[{"given":"Andrew","family":"Slack","sequence":"first","affiliation":[{"name":"King\u2019s College Hospital, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Tredger","sequence":"additional","affiliation":[{"name":"King\u2019s College Hospital, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nigel","family":"Brown","sequence":"additional","affiliation":[{"name":"King\u2019s College Hospital, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Corcoran","sequence":"additional","affiliation":[{"name":"King\u2019s College Hospital, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin","family":"Moore","sequence":"additional","affiliation":[{"name":"Royal Free Hospital, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2013,7,11]]},"reference":[{"key":"bibr1-0004563213487715","doi-asserted-by":"publisher","DOI":"10.1002\/lt.20017"},{"key":"bibr2-0004563213487715","doi-asserted-by":"publisher","DOI":"10.1002\/lt.22128"},{"key":"bibr3-0004563213487715","doi-asserted-by":"publisher","DOI":"10.1002\/lt.20994"},{"key":"bibr4-0004563213487715","doi-asserted-by":"publisher","DOI":"10.1053\/he.2000.5852"},{"key":"bibr5-0004563213487715","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/13.5.1176"},{"key":"bibr6-0004563213487715","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.V92310"},{"key":"bibr7-0004563213487715","doi-asserted-by":"publisher","DOI":"10.1258\/000456307780945804"},{"key":"bibr8-0004563213487715","first-page":"2738","volume":"88","author":"Nilsson-Ehle P","year":"1991","journal-title":"Lakartidningen"},{"key":"bibr9-0004563213487715","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2004.11.046"},{"key":"bibr10-0004563213487715","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2011.405"},{"key":"bibr11-0004563213487715","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0801209"},{"key":"bibr12-0004563213487715","doi-asserted-by":"publisher","DOI":"10.1023\/A:1007669411738"},{"key":"bibr13-0004563213487715","unstructured":"Guideline on Validation of Bioanalytical Methods: European Medicines Agency\n                      ; 2009. 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