{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T15:30:05Z","timestamp":1789399805996,"version":"build-2803163510"},"reference-count":29,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2013,7,30]],"date-time":"2013-07-30T00:00:00Z","timestamp":1375142400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Ann Clin Biochem"],"published-print":{"date-parts":[[2014,3]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Neonatal screening for haemoglobin (Hb) disorders is a standard of care in several developed countries with the main objective to detect Hb S. Such practice has not been established in Thailand where \u03b1-thalassaemia and haemoglobin E (Hb E) are highly prevalent. Early identification of thalassaemias could be helpful and strengthen the programme for prevention and control for severe thalassaemias.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Data from isoelectric focusing (IEF) and Isoscan\u00ae for detecting types and amount (%) of each haemoglobin in 350 newborn\u2019s dried blood spots were analysed and compared with the comprehensive genotype analysis by DNA studies as a gold standard.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Based on genetic profiles, there were 10 different categories: (1) normal ( n\u2009=\u2009227), (2) \u03b1\n                      <jats:sup>+<\/jats:sup>\n                      -thalassaemia trait ( n\u2009=\u200914), (3) \u03b1\n                      <jats:sup>0<\/jats:sup>\n                      -thalassaemia trait ( n\u2009=\u200913), (4) \u03b2\n                      <jats:sup>0<\/jats:sup>\n                      -thalassaemia trait ( n\u2009=\u20097), (5) Hb E trait ( n\u2009=\u200972), (6) Hb E trait with \u03b1\n                      <jats:sup>0<\/jats:sup>\n                      -thalassaemia or homozygous \u03b1\n                      <jats:sup>+<\/jats:sup>\n                      -thalassaemia ( n\u2009=\u20095), (7) Hb E trait with \u03b1\n                      <jats:sup>+<\/jats:sup>\n                      -thalassaemia trait ( n\u2009=\u20095), (8) homozygous Hb E ( n\u2009=\u20093), (9) homozygous Hb E with \u03b1\n                      <jats:sup>0<\/jats:sup>\n                      -thalassaemia trait ( n\u2009=\u20091) and (10) Hb H disease ( n\u2009=\u20093). The presence of Hb Bart\u2019s and Hb E were used to identify cases with \u03b1-thalassaemia and Hb E, respectively. We set 0.25% of Hb Bart\u2019s and 1.5% of Hb E as a cut-off level to detect \u03b1\n                      <jats:sup>+<\/jats:sup>\n                      -thalassaemia trait (sensitivity 92.86% and specificity 74.0%) and Hb E trait with 100% of both sensitivity and specificity for IEF diagnosis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Although molecular diagnosis seems to be better for definitive diagnosis of thalassaemia syndromes at birth, however, using our reference range described herein, IEF can be applied in a resource-limiting setting with acceptable reliability.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/0004563213491078","type":"journal-article","created":{"date-parts":[[2013,7,30]],"date-time":"2013-07-30T23:43:59Z","timestamp":1375227839000},"page":"237-247","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":19,"title":["Diagnostic applications of newborn screening for \u03b1-thalassaemias, haemoglobins E and H disorders using isoelectric focusing on dry blood spots"],"prefix":"10.1177","volume":"51","author":[{"given":"Punyanuch","family":"Jindatanmanusan","sequence":"first","affiliation":[{"name":"Hematology Division, Phramongkutklao Hospital, Bangkok, Thailand"},{"name":"Department of Clinical Pathology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suchada","family":"Riolueang","sequence":"additional","affiliation":[{"name":"Department of Immunology, Siriraj Hospital Mahidol University, Bangkok, Thailand"},{"name":"Thalassemia Centre, Siriraj Hospital, Mahidol University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Waraporn","family":"Glomglao","sequence":"additional","affiliation":[{"name":"Division of Hematology\/Oncology, Siriraj Hospital, Mahidol University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaowapa","family":"Sukontharangsri","sequence":"additional","affiliation":[{"name":"Department of Obstetrics & Gynaecology, Phramongkutklao Hospital, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sangkae","family":"Chamnanvanakij","sequence":"additional","affiliation":[{"name":"Hematology Division, Phramongkutklao Hospital, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kitti","family":"Torcharus","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology, Phramongkutklao Hospital, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vip","family":"Viprakasit","sequence":"additional","affiliation":[{"name":"Thalassemia Centre, Siriraj Hospital, Mahidol University, Bangkok, Thailand"},{"name":"Division of Hematology\/Oncology, Siriraj Hospital, Mahidol University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2013,7,30]]},"reference":[{"key":"bibr1-0004563213491078","doi-asserted-by":"publisher","DOI":"10.1002\/9780470696705"},{"key":"bibr2-0004563213491078","first-page":"1","volume-title":"National conference on thalassemia and hemoglobinopathies","author":"Teinthavorn V","year":"2006"},{"key":"bibr3-0004563213491078","doi-asserted-by":"crossref","unstructured":"Viprakasit V, Limwongse C, Sukpanichnant S, et\u00a0al. 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