{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T07:53:16Z","timestamp":1791445996653,"version":"4.3.3"},"reference-count":24,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2014,8,29]],"date-time":"2014-08-29T00:00:00Z","timestamp":1409270400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Ann Clin Biochem"],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Immunometric assays have recently shown higher specificity in the detection of human insulin than radioimmunoassays with almost no cross-reaction with proinsulin or C peptide. The introduction of the new insulin analogues on the market, however, has raised the need to define their cross-reactivity in these assays. Several studies have been published in this regard with different results.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The analogues studied were insulins lispro, aspart, glargine, detemir, and glulisine. Insulin concentrations were measured in Immulite\u00ae 2000 and Advia Centaur\u00ae XP (Siemens Healthcare Diagnostics), and Elecsys\u00ae Modular Analytics E170 (Roche). All samples were processed 15 times in the same analytical run following a random sequence. Those samples which showed statistically and clinically significant changes in insulin concentration were reprocessed using increasing concentrations of analogue, and this was done twice, using two different serum pools, one with a low concentration of insulin and one with a high concentration of insulin.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>In the Elecsys\u00ae E170 analyser, glargine showed statistical changes (comparison of mean concentrations with p\u2009&lt;\u20090.05) and clinically significant changes in measured insulin (percentage difference 986.2%\u2009&gt;\u2009reference change value: 59.8%), and the interference increased with increasing concentrations of analogue; the differences were not significant in the case of the other analogues. In the Advia Centaur\u00ae and Immulite\u00ae 2000 only the results for glulisine did not present significance (percentage difference 44.7%\u2009&lt;\u2009reference change value 103.5%). Increasing concentrations of aspart, glargine, and lispro showed increased interference in Immulite\u00ae 2000.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>In the Elecsys\u00ae E170 assay, relevant cross-reactivity was only detected with insulin glargine, whereas in the other analysers all analogues except glulisine showed significant interference.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/0004563214551613","type":"journal-article","created":{"date-parts":[[2014,8,29]],"date-time":"2014-08-29T21:54:23Z","timestamp":1409349263000},"page":"312-318","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":30,"title":["Cross-reactivity of insulin analogues with three insulin assays"],"prefix":"10.1177","volume":"52","author":[{"given":"A","family":"Dayaldasani","sequence":"first","affiliation":[{"name":"General Laboratory, Regional University Hospital, M\u00e1laga, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Rodr\u00edguez Espinosa","sequence":"additional","affiliation":[{"name":"General Laboratory, Regional University Hospital, M\u00e1laga, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P","family":"Oc\u00f3n S\u00e1nchez","sequence":"additional","affiliation":[{"name":"General Laboratory, Regional University Hospital, M\u00e1laga, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V","family":"P\u00e9rez Valero","sequence":"additional","affiliation":[{"name":"General Laboratory, Regional University Hospital, M\u00e1laga, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2014,8,29]]},"reference":[{"key":"bibr1-0004563214551613","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-011-2336-9"},{"key":"bibr2-0004563214551613","unstructured":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Diabetes y Enfermedades Metab\u00f3licas Asociadas, Sociedad Espa\u00f1ola de Diabetes. Prevalencia de la Diabetes en Espa\u00f1a: Estudio di@bet.es. [internet], http:\/\/www.adc.cat\/not\/not-271.pdf (2012, accessed 08 July 2012)."},{"key":"bibr3-0004563214551613","doi-asserted-by":"publisher","DOI":"10.1016\/j.diabet.2006.12.002"},{"key":"bibr4-0004563214551613","doi-asserted-by":"publisher","DOI":"10.1258\/000456306777695690"},{"key":"bibr5-0004563214551613","doi-asserted-by":"publisher","DOI":"10.1590\/S0004-27302007000300022"},{"key":"bibr6-0004563214551613","first-page":"266","volume":"22","author":"Serra Sansone MP","year":"2006","journal-title":"Rev Med Urug"},{"key":"bibr7-0004563214551613","doi-asserted-by":"publisher","DOI":"10.1089\/dia.2011.0038"},{"key":"bibr8-0004563214551613","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2008.118380"},{"key":"bibr9-0004563214551613","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201300300"},{"key":"bibr10-0004563214551613","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2003.026625"},{"key":"bibr11-0004563214551613","doi-asserted-by":"publisher","DOI":"10.1258\/acb.2010.010002"},{"key":"bibr12-0004563214551613","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/47.3.602"},{"key":"bibr13-0004563214551613","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2006.077784"},{"key":"bibr14-0004563214551613","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-5491.2009.02815.x"},{"key":"bibr15-0004563214551613","doi-asserted-by":"publisher","DOI":"10.4158\/EP.14.8.1006"},{"key":"bibr16-0004563214551613","first-page":"449","volume":"11","author":"Andres R","year":"1992","journal-title":"Quim Clin"},{"key":"bibr17-0004563214551613","volume-title":"Interference testing in clinical chemistry. Approved Guideline \u2013 2nd ed. CLSI document EP7-A2 (ISBN 1-56238-584-4)","author":"Clinical and Laboratory Standards Institute (CLSI)","year":"2005"},{"key":"bibr18-0004563214551613","first-page":"4","volume":"1","author":"Ozturk","year":"2012","journal-title":"Biochem Anal Biochem"},{"key":"bibr19-0004563214551613","unstructured":"Sociedad Espa\u00f1ola de Bioqu\u00edmica Cl\u00ednica y Patolog\u00eda Molecular. Tabla de Variaci\u00f3n Biol\u00f3gica \u2013 especificaciones deseables. [internet], http:\/\/www.seqc.es\/es\/Sociedad\/7\/51\/102\/Base_de_datos_de_Variacion_biologica_Bases_de_datos_y_documentos_del_Comite_de_Garantia_de_la_Calidad_y_Acreditacion_Comite_de_Garantia_de_la_Calidad_y_Acreditacion_\/ (2012, accessed 12 July 2012)."},{"key":"bibr20-0004563214551613","doi-asserted-by":"publisher","DOI":"10.1515\/cclm-2013-0427"},{"key":"bibr21-0004563214551613","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2006.082214"},{"key":"bibr22-0004563214551613","doi-asserted-by":"publisher","DOI":"10.3343\/kjlm.2011.31.1.22"},{"key":"bibr23-0004563214551613","first-page":"94","volume":"6","author":"Abell\u00e1n Tejada L","year":"2013","journal-title":"Rev Lab Clin"},{"key":"bibr24-0004563214551613","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2005.065995"}],"container-title":["Annals of Clinical Biochemistry: International Journal of Laboratory Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0004563214551613","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/0004563214551613","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0004563214551613","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T12:21:56Z","timestamp":1777378916000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/0004563214551613"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,8,29]]},"references-count":24,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2015,5]]}},"alternative-id":["10.1177\/0004563214551613"],"URL":"https:\/\/doi.org\/10.1177\/0004563214551613","relation":{},"ISSN":["0004-5632","1758-1001"],"issn-type":[{"value":"0004-5632","type":"print"},{"value":"1758-1001","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,8,29]]}}}