{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T03:54:53Z","timestamp":1790654093858,"version":"4.1.0"},"reference-count":17,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2015,4,10]],"date-time":"2015-04-10T00:00:00Z","timestamp":1428624000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Ann Clin Biochem"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Analysis of lipoprotein profile gives important clinical information for lipid-lowering therapy which prevents atherosclerotic diseases. The lipoprotein classes can be isolated from serum with ultracentrifugation, which inevitably consumes a long time and needs large serum volume. We have established a method with anion-exchange chromatography with 1.0\u2009\u00b5L of the injected volume in 5.2\u2009min for assay of one sample.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>One-hundred-forty-one male volunteers without overt diseases were divided three groups (Group 1, non-dyslipidemia with LDL-cholesterol [LDL-C] &lt;120\u2009mg\/dL and HDL-cholesterol (HDL-C) \u226540\u2009mg\/dL; Group 2, borderline dyslipidemia with 120\u2009\u2264\u2009LDL-C\u2009&lt;\u2009140\u2009mg\/dL and HDL-C \u226540\u2009mg\/dL; Group 3, dyslipidemia with LDL-C\u2009\u2265\u2009140\u2009mg\/dL or HDL-C\u2009&lt;\u200940\u2009mg\/dL). Their lipoprotein profiles were evaluated by rapid anion-exchange chromatography, which measured concentrations of HDL-C, LDL-C, IDL-cholesterol, VLDL-cholesterol, and other fraction (chylomicron + lipoprotein [a])-cholesterol (other-C).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The within-day and between-day assay coefficients of variation of lipoprotein cholesterol values were 0.33\u20134.31% and 2.37\u20139.19%, respectively. The correlation coefficients between values of HDL-C, LDL-C, IDL-C and VLDL-C by the anion-exchange chromatography and those by ultracentrifugal method were 0.97, 0.92, 0.58 and 0.94, respectively. Group 3 had significantly lower HDL-C and higher concentrations of IDL-C, VLDL-C and other-C than did Group 1. Group 2, borderline dyslipidemia, had significantly higher concentrations of IDL-C and VLDL-C than did Group 1.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The rapid anion-exchange chromatography assay may be sufficiently applied to the assessment of borderline dyslipidemia.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/0004563215584405","type":"journal-article","created":{"date-parts":[[2015,4,10]],"date-time":"2015-04-10T21:32:37Z","timestamp":1428701557000},"page":"638-646","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":15,"title":["A rapid anion-exchange chromatography for measurement of cholesterol concentrations in five lipoprotein classes and estimation of lipoprotein profiles in male volunteers without overt diseases"],"prefix":"10.1177","volume":"52","author":[{"given":"Daisuke","family":"Manita","sequence":"first","affiliation":[{"name":"Bioscience Division, TOSOH Corporation, Kanagawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuji","family":"Hirowatari","sequence":"additional","affiliation":[{"name":"Laboratory Science, Department of Health Science, Saitama Prefectural University, Saitama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Jikei University Kashiwa Hospital, Chiba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2015,4,10]]},"reference":[{"key":"bibr1-0004563215584405","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1987.03390160062027"},{"key":"bibr2-0004563215584405","doi-asserted-by":"publisher","DOI":"10.1016\/j.ypmed.2011.02.019"},{"key":"bibr3-0004563215584405","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(77)90874-9"},{"key":"bibr4-0004563215584405","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa064278"},{"key":"bibr5-0004563215584405","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2796.1999.00548.x"},{"key":"bibr6-0004563215584405","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjcard.2006.06.032"},{"key":"bibr7-0004563215584405","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9150(98)00246-9"},{"key":"bibr8-0004563215584405","first-page":"891","volume":"43","author":"Beaumont JL","year":"1970","journal-title":"Bull World Health Organ"},{"key":"bibr9-0004563215584405","doi-asserted-by":"publisher","DOI":"10.1172\/JCI103182"},{"key":"bibr10-0004563215584405","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.D300003-JLR200"},{"key":"bibr11-0004563215584405","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.D003624"},{"key":"bibr12-0004563215584405","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijcard.2013.06.023"},{"key":"bibr13-0004563215584405","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)42680-X"},{"key":"bibr14-0004563215584405","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.s1-5.1.32"},{"key":"bibr15-0004563215584405","doi-asserted-by":"publisher","DOI":"10.5551\/jat.12.98"},{"key":"bibr16-0004563215584405","doi-asserted-by":"publisher","DOI":"10.1161\/hq0701.092246"},{"key":"bibr17-0004563215584405","doi-asserted-by":"publisher","DOI":"10.1016\/j.cccn.2004.04.018"}],"container-title":["Annals of Clinical Biochemistry: International Journal of Laboratory Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0004563215584405","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/0004563215584405","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0004563215584405","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T12:22:17Z","timestamp":1777378937000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/0004563215584405"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,4,10]]},"references-count":17,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2015,11]]}},"alternative-id":["10.1177\/0004563215584405"],"URL":"https:\/\/doi.org\/10.1177\/0004563215584405","relation":{},"ISSN":["0004-5632","1758-1001"],"issn-type":[{"value":"0004-5632","type":"print"},{"value":"1758-1001","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,4,10]]}}}