{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T06:27:20Z","timestamp":1777444040439,"version":"3.51.4"},"reference-count":16,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2015,12,17]],"date-time":"2015-12-17T00:00:00Z","timestamp":1450310400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Ann Clin Biochem"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The presence of C3-epimer (C-3-epi-25-hydroxyvitamin D) in infant serum may complicate 25-hydroxyvitamin D (25(OH)D) measurement when using liquid chromatography tandem mass spectrometry assays that do not separately measure the epimer. We measured the concentration of C3-epi-25(OH)D in neonatal samples in Western Australian using umbilical cord blood samples and a liquid chromatography tandem mass spectrometry assay that separately quantifies 25(OH)D and C3-epi-25(OH)D.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A total of 120 anonymized cord blood samples were analysed using a liquid chromatography tandem mass spectrometry assay that utilizes two CSH fluoro-phenyl columns in series. Chromatography was performed on a Waters Acquity Ultra Performance Liquid system, and quantification was using a Waters Quattro Premier XE mass spectrometer.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      C3-epi-25(OH)D\n                      <jats:sub>3<\/jats:sub>\n                      was detected in all umbilical cord blood samples (median 5.2\u2009nmol\/L, IQR 3.7\u20136.6\u2009nmol\/L) and contributed 6.6% (SD 2.6, 95% CI [6.1, 7.1]) of the total 25(OH)D concentration. Mean 25(OH)D\n                      <jats:sub>3<\/jats:sub>\n                      measured in cord blood was 79.1\u2009nmol\/L (SD 22.7\u2009nmol\/L). A positive relationship (R\n                      <jats:sup>2\u2009<\/jats:sup>\n                      =\u20090.35, P\u2009&lt;\u20090.0005) between 25(OH)D\n                      <jats:sub>3<\/jats:sub>\n                      levels and C3-epi-25(OH)D\n                      <jats:sub>3<\/jats:sub>\n                      was noted in this cohort. No samples contained 25(OH)D\n                      <jats:sub>2<\/jats:sub>\n                      or C3-epi-25(OH)D\n                      <jats:sub>2<\/jats:sub>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      C3-epi-25(OH)D\n                      <jats:sub>3<\/jats:sub>\n                      is present in all neonatal samples but contributes &lt;10% of the total 25(OH)D concentration which is unlikely to be clinically significant. Liquid chromatography tandem mass spectrometry assays that do not separately quantify C3-epi-25(OH)D\n                      <jats:sub>3<\/jats:sub>\n                      from other vitamin D metabolites may potentially overestimate neonatal 25(OH)D levels, but diagnostic misclassification in neonates is unlikely.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/0004563215625693","type":"journal-article","created":{"date-parts":[[2015,12,18]],"date-time":"2015-12-18T21:36:25Z","timestamp":1450474585000},"page":"593-598","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":22,"title":["25-Hydroxyvitamin D C3-epimer is universally present in neonatal Western Australian samples but is unlikely to contribute to diagnostic misclassification"],"prefix":"10.1177","volume":"53","author":[{"given":"David J","family":"Cooke","sequence":"first","affiliation":[{"name":"School of Medicine and Pharmacology, University of Western Australia, Perth, Western Australia, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian R","family":"Cooke","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry, Pathwest Royal Perth Hospital, Perth, Western Australia, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Damon A","family":"Bell","sequence":"additional","affiliation":[{"name":"School of Medicine and Pharmacology, University of Western Australia, Perth, Western Australia, Australia"},{"name":"Department of Clinical Biochemistry, Pathwest Royal Perth Hospital, Perth, Western Australia, Australia"},{"name":"Clinical Biochemistry, St John of God Pathology, Western Australia, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samuel D","family":"Vasikaran","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry, Pathwest Royal Perth Hospital, Perth, Western Australia, Australia"},{"name":"School of Pathology and Laboratory Medicine, University of Western Australia, Perth, Western Australia, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Glendenning","sequence":"additional","affiliation":[{"name":"School of Medicine and Pharmacology, University of Western Australia, Perth, Western Australia, Australia"},{"name":"Department of Clinical Biochemistry, Pathwest Royal Perth Hospital, Perth, Western Australia, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2015,12,17]]},"reference":[{"key":"bibr1-0004563215625693","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2010.02.025"},{"key":"bibr2-0004563215625693","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjmed.2003.12.029"},{"key":"bibr3-0004563215625693","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-010-3993-0"},{"key":"bibr4-0004563215625693","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2004-0682"},{"key":"bibr5-0004563215625693","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/88.2.507S"},{"key":"bibr6-0004563215625693","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2012.10.037"},{"key":"bibr7-0004563215625693","doi-asserted-by":"publisher","DOI":"10.1016\/j.steroids.2010.02.012"},{"key":"bibr8-0004563215625693","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/77.2.522"},{"key":"bibr9-0004563215625693","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2006-0710"},{"key":"bibr10-0004563215625693","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2011.09.028"},{"key":"bibr11-0004563215625693","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(99)00377-4"},{"key":"bibr12-0004563215625693","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2011.050"},{"key":"bibr13-0004563215625693","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2891-12-77"},{"key":"bibr14-0004563215625693","first-page":"1","volume":"1","author":"G\u00f6ren 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