{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T14:13:03Z","timestamp":1781100783505,"version":"3.54.1"},"reference-count":28,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2016,8,5]],"date-time":"2016-08-05T00:00:00Z","timestamp":1470355200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Ann Clin Biochem"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>We aimed to determine whether the discrepancy between haemoglobin A1c values determined by high-performance liquid chromatography and enzymatic haemoglobin A1c measurements in diabetic patients was clinically relevant.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We randomly recruited 1421 outpatients undergoing diabetic treatment and follow-up who underwent at least three haemoglobin A1c measurements between April 2014 and March 2015 at our clinic. In 6369 samples, haemoglobin A1c was simultaneously measured by HA-8160 and MetaboLead (enzymatic assay), and the values were compared.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>haemoglobin A1c measurements by high-performance liquid chromatography and enzymatic assay were strongly correlated (correlation coefficient: 0.9828, linear approximation curve y\u2009=\u20090.9986x\u2009\u2212\u20090.2507). Mean haemoglobin A1c (6.8\u2009\u00b1\u20091.0%) measured by high-performance liquid chromatography was significantly higher than that measured by enzymatic assay (6.5\u2009\u00b1\u20091.0%, P\u2009&lt;\u20090.0001). During the sample processing, four (0.3%) subjects presented consistently lower haemoglobin A1c values (&lt;0.7%) by high-performance liquid chromatography than those from enzymatic assay. Of these, three had Hb Toranomon [\u03b2112 (G14) Cys\u2192Trp]. The fourth had Hb Ube-2 [\u03b168 (E17) Asn\u2192Asp]. One other subject presented consistently higher haemoglobin A1c values (&gt;1%) by high-performance liquid chromatography than those from enzymatic assay and was diagnosed with a \u221277 (T\u2009&gt;\u2009C) mutation in the \u03b4-globin gene. These unrelated asymptomatic subjects had normal erythrocyte profiles, without anaemia.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>We showed that haemoglobin A1c values measured by high-performance liquid chromatography were significantly higher than those measured by enzymatic assay in diabetic subjects. However, when an oversized deviation (&gt;0.7%) between glycaemic control status and haemoglobin A1c is apparent, clinicians should check the methods used to measure haemoglobin A1c and consider the possible presence of a haemoglobin variant.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/0004563216664366","type":"journal-article","created":{"date-parts":[[2016,8,5]],"date-time":"2016-08-05T20:30:24Z","timestamp":1470429024000},"page":"432-437","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Haemoglobin variants may cause significant differences in haemoglobin A1c as measured by high-performance liquid chromatography and enzymatic methods in diabetic patients: a cross-sectional study"],"prefix":"10.1177","volume":"54","author":[{"given":"Shuichi","family":"Otabe","sequence":"first","affiliation":[{"name":"Division of Endocrinology and Metabolism, Department of Medicine, Kurume University 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