{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T06:32:17Z","timestamp":1777444337004,"version":"3.51.4"},"reference-count":35,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2018,1,30]],"date-time":"2018-01-30T00:00:00Z","timestamp":1517270400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Ann Clin Biochem"],"published-print":{"date-parts":[[2018,9]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Several approaches exist to screen neonates for congenital cytomegalovirus infection. We here describe a new method using cellulose pads for urine collection and its evaluation in an experimental and a clinical setting.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We systematically tested the effect of storage duration of the pads after exposure to cytomegalovirus-positive urine, meconium contamination and specimen handling on the cytomegalovirus load and the detection rate. Further, the method was tested in clinical practice in a cohort of 500 neonates.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Following exposure of urine pads with cytomegalovirus-positive urine, the viral load decreased after 15 min, 12 h, 24 h, and 7 days to 63.2%, 42.1%, 31.6%, and 9.3% of the baseline value. Cytomegalovirus detection rate after seven days was 100%. Contamination with meconium resulted in a comparable reduction of the viral load. The detection rate for dried urine pads after seven days was 93.3%. In clinical practice, urine collection from pads was successful in 73.6% by the first attempt and in 26.4% by the second attempt.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Urine collection using cellulose pads seems feasible regardless of a reduction of the cytomegalovirus load due to exposure to the pad itself or to meconium. Drying of the exposed urine pad should be avoided.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/0004563217750183","type":"journal-article","created":{"date-parts":[[2018,8,20]],"date-time":"2018-08-20T10:28:03Z","timestamp":1534760883000},"page":"553-560","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Evaluation of cellulose pads as a method to detect cytomegalovirus DNA in neonatal urine"],"prefix":"10.1177","volume":"55","author":[{"given":"Florian","family":"Kipfmueller","sequence":"first","affiliation":[{"name":"Department of Neonatology and Pediatric Critical Care Medicine, University Children\u2019s Hospital Bonn, Bonn, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kira","family":"Jungbluth","sequence":"additional","affiliation":[{"name":"Department of Neonatology and Pediatric Critical Care Medicine, University Children\u2019s Hospital Bonn, Bonn, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna M","family":"Eis-H\u00fcbinger","sequence":"additional","affiliation":[{"name":"Institute of Virology, University of Bonn Medical Center, Bonn, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrike","family":"Reber","sequence":"additional","affiliation":[{"name":"Institute of Virology, University of Bonn Medical Center, Bonn, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Holdenrieder","sequence":"additional","affiliation":[{"name":"Institute for Clinical Chemistry and Clinical Pharmacology, University of Bonn Medical Center, Bonn, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrich","family":"Gembruch","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, University of Bonn Medical Centre, Bonn, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Bartmann","sequence":"additional","affiliation":[{"name":"Department of Neonatology and Pediatric Critical Care Medicine, University Children\u2019s Hospital Bonn, Bonn, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas","family":"Mueller","sequence":"additional","affiliation":[{"name":"Department of Neonatology and Pediatric Critical Care Medicine, University Children\u2019s Hospital Bonn, Bonn, 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