{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T11:40:32Z","timestamp":1778499632810,"version":"3.51.4"},"reference-count":7,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2018,6,7]],"date-time":"2018-06-07T00:00:00Z","timestamp":1528329600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Ann Clin Biochem"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Faecal immunochemical tests (FIT) for haemoglobin (Hb) are being used in the investigation of colorectal cancer. These tests use antibodies raised to the globin moiety of human Hb. Where the globin structure is abnormal or reduced, it is possible that antibody binding, and thus Hb-detection may be affected.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Lysates prepared from whole blood samples of patients with known variants were diluted in manufacturer-specific buffer to 10, 100 and 500 \u03bcg Hb\/g faeces. These samples were analysed on four FIT analysers and the results compared with samples with no known variant present (normal samples).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The results from this study show that of 20 variants tested, three showed a decrease in detection by all four analysers. These were \u03b2-thalassaemia major and two fetal cord blood samples.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Of 20 common Hb variants studied, 17 did not affect detection of Hb by the FIT systems tested. Hb variants leading to a reduction in the presence of a globin chain caused a reduction in Hb detection; in such cases, cancers could be missed.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/0004563218778716","type":"journal-article","created":{"date-parts":[[2018,5,23]],"date-time":"2018-05-23T23:48:29Z","timestamp":1527119309000},"page":"706-709","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":7,"title":["An assessment of the effect of haemoglobin variants on detection by faecal immunochemical tests"],"prefix":"10.1177","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6886-5631","authenticated-orcid":false,"given":"Magdalen RR","family":"Carroll","sequence":"first","affiliation":[{"name":"NHS Bowel Cancer Screening Programme Southern Hub, Royal Surrey County Hospital, Guildford, UK"},{"name":"Berkshire and Surrey Pathology Services, Royal Surrey County Hospital, Guildford, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cerin","family":"John","sequence":"additional","affiliation":[{"name":"NHS Bowel Cancer Screening Programme Southern Hub, Royal Surrey County Hospital, Guildford, UK"},{"name":"Berkshire and Surrey Pathology Services, Royal Surrey County Hospital, Guildford, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Debbie","family":"Mantio","sequence":"additional","affiliation":[{"name":"Haemoglobinopathy Screening Laboratory, The Doctors Laboratory, Central Middlesex Hospital-->, London, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natasha K","family":"Djedovic","sequence":"additional","affiliation":[{"name":"Northwick Park Hospital, NHS Bowel Cancer Screening Programme London Hub, London, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sally C","family":"Benton","sequence":"additional","affiliation":[{"name":"NHS Bowel Cancer Screening Programme Southern Hub, Royal Surrey County Hospital, Guildford, UK"},{"name":"Berkshire and Surrey Pathology Services, Royal Surrey County Hospital, Guildford, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2018,6,7]]},"reference":[{"key":"bibr1-0004563218778716","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2014-309086"},{"key":"bibr2-0004563218778716","unstructured":"National Institute for Health and Care Excellence. Quantitative faecal immunochemical tests to guide referral for colorectal cancer in primary care. Guidance and guidelines. NICE, www.nice.org.uk\/guidance\/dg30 (2017, accessed 14 November 2017)."},{"key":"bibr3-0004563218778716","doi-asserted-by":"publisher","DOI":"10.5217\/ir.2016.14.1.5"},{"key":"bibr4-0004563218778716","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a011858"},{"key":"bibr5-0004563218778716","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-03-325258"},{"key":"bibr6-0004563218778716","unstructured":"SENTiFIT\u00ae FOB Gold\u00ae Latex (package insert). Milan, Italy: SENTINEL Diagnostics, 2012."},{"key":"bibr7-0004563218778716","doi-asserted-by":"publisher","DOI":"10.5301\/jbm.5000150"}],"container-title":["Annals of Clinical Biochemistry: International Journal of Laboratory Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0004563218778716","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/0004563218778716","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0004563218778716","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T12:23:33Z","timestamp":1777379013000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/0004563218778716"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,6,7]]},"references-count":7,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2018,11]]}},"alternative-id":["10.1177\/0004563218778716"],"URL":"https:\/\/doi.org\/10.1177\/0004563218778716","relation":{},"ISSN":["0004-5632","1758-1001"],"issn-type":[{"value":"0004-5632","type":"print"},{"value":"1758-1001","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,6,7]]}}}