{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T01:17:08Z","timestamp":1789953428645,"version":"4.0.1"},"reference-count":19,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2018,6,11]],"date-time":"2018-06-11T00:00:00Z","timestamp":1528675200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Ann Clin Biochem"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Congenital disorders of glycosylation (CDG) are a growing group of rare genetic disorders. The most frequently used screening method is sialotransferrin profiling using isoelectric focusing (IEF). Capillary zone electrophoresis (CZE) may be a simple and fast alternative. We investigated the Capillarys\u2122 CDT assay (Sebia, France) to screen for N-glycosylation disorders, using IEF as gold standard.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Intra- and inter-assay precision were established, and analyses in heparin-anticoagulated plasma and serum were compared. Accuracy was assessed by comparing IEF and CZE profiles of 153 samples, including 49 normal, 53 CDG type I, 2 CDG type II, 1 combined CDG type I and type II and 48 samples with a Tf-polymorphism. Neuraminidase-treated plasma was analysed to discriminate CDG and Tf-polymorphisms using samples of 52 subjects (25 had a confirmed Tf-polymorphism). Age-dependent reference values were established using profiles of 312 samples.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Heparin-plasma is as suitable as serum for CDG screening with the Capillarys\u2122 CDT assay. The precision of the method is high, with a limit of quantification (LOQ) of 0.5%. All profiles, including CDG and Tf-polymorphisms, were correctly identified with CZE. Forty-nine of 52 neuraminidase-treated samples correctly identified the presence\/absence of a Tf-polymorphism. Interferences in 3\/52 samples hampered interpretation. Sialo-Tf profiles were dependent of age, in particular in the first three months of age.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>CZE analysis with the Capillarys\u2122 CDT kit (Sebia) is a fast and reliable method for screening of N-glycosylation defects. Tf-polymorphisms could be excluded after overnight incubation with neuraminidase.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/0004563218779609","type":"journal-article","created":{"date-parts":[[2018,5,23]],"date-time":"2018-05-23T23:48:29Z","timestamp":1527119309000},"page":"693-701","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":13,"title":["Fast screening of N-glycosylation disorders by sialotransferrin profiling with capillary zone electrophoresis"],"prefix":"10.1177","volume":"55","author":[{"given":"HA","family":"Kingma","sequence":"first","affiliation":[{"name":"Laboratory of Metabolic Diseases, Department of Laboratory Medicine, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"FH","family":"van der Sluijs","sequence":"additional","affiliation":[{"name":"Laboratory of Metabolic Diseases, Department of Laboratory Medicine, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3477-0770","authenticated-orcid":false,"given":"MR","family":"Heiner-Fokkema","sequence":"additional","affiliation":[{"name":"Laboratory of Metabolic Diseases, Department of Laboratory Medicine, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2018,6,11]]},"reference":[{"key":"bibr1-0004563218779609","first-page":"30494","volume":"1769","author":"P\u00e9anne R","year":"2017","journal-title":"Eur J Med Genet"},{"key":"bibr2-0004563218779609","doi-asserted-by":"publisher","DOI":"10.1007\/s10545-017-0050-6"},{"key":"bibr3-0004563218779609","doi-asserted-by":"publisher","DOI":"10.1007\/s10545-014-9720-9"},{"key":"bibr4-0004563218779609","doi-asserted-by":"publisher","DOI":"10.1007\/s10719-015-9639-x"},{"key":"bibr5-0004563218779609","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201700914"},{"key":"bibr6-0004563218779609","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2003.021568"},{"key":"bibr7-0004563218779609","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.22167"},{"key":"bibr8-0004563218779609","doi-asserted-by":"publisher","DOI":"10.1007\/BF01053199"},{"key":"bibr9-0004563218779609","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150181017"},{"key":"bibr10-0004563218779609","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2009.10.004"},{"key":"bibr11-0004563218779609","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2009.06.031"},{"key":"bibr12-0004563218779609","doi-asserted-by":"publisher","DOI":"10.1080\/00365510601004077"},{"key":"bibr13-0004563218779609","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150130173"},{"key":"bibr14-0004563218779609","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201700211"},{"key":"bibr15-0004563218779609","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2010.07.015"},{"key":"bibr16-0004563218779609","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/48.12.2177"},{"key":"bibr17-0004563218779609","doi-asserted-by":"publisher","DOI":"10.1007\/s00414-015-1162-x"},{"key":"bibr18-0004563218779609","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2003.019240"},{"key":"bibr19-0004563218779609","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2014.08.011"}],"container-title":["Annals of Clinical Biochemistry: International Journal of Laboratory Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0004563218779609","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/0004563218779609","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0004563218779609","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T12:23:34Z","timestamp":1777379014000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/0004563218779609"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,6,11]]},"references-count":19,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2018,11]]}},"alternative-id":["10.1177\/0004563218779609"],"URL":"https:\/\/doi.org\/10.1177\/0004563218779609","relation":{},"ISSN":["0004-5632","1758-1001"],"issn-type":[{"value":"0004-5632","type":"print"},{"value":"1758-1001","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,6,11]]}}}