{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T06:33:01Z","timestamp":1777444381972,"version":"3.51.4"},"reference-count":33,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2018,6,11]],"date-time":"2018-06-11T00:00:00Z","timestamp":1528675200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Ann Clin Biochem"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Liquid chromatography tandem mass spectrometry (LC-MS\/MS) enables specific and sensitive quantification of steroids with a high throughput. Saliva sampling is advantageous for multisample profiling over longer periods of time, as it is non-invasive, cheap, can be carried out at home and does not require the attendance of clinical personnel. We developed a rapid LC-MS\/MS for the measurement of salivary progesterone, frequently assessed as ovulation marker in patients desiring fertility.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Samples (300 \u03bcL) were prepared by supported liquid extraction using dichloromethane and were reconstituted in 40% methanol. Chromatography was performed using a C8 column with a water\/methanol gradient containing 0.1% formic acid and 2 mmol\/L ammonium acetate. Quantification was performed with a Waters TQ-S mass spectrometer.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Total run time was 5.5 min. The lower limit of quantification was 20\u2009pmol\/L (1.2 fmol on column). Inter- and intra-assay comparison showed coefficients of variation and bias between measured and nominal concentrations of less than 11%. Mean recovery was 91%. Interference with a large set of natural and synthetic steroids was excluded. The assay was successfully applied to measure progesterone variation during the menstrual cycle ( n\u2009=\u20099) and diurnal variations during luteal phase ( n\u2009=\u20097) in regularly cycling women.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>We present a novel LC-MS\/MS assay for the determination of salivary progesterone with high-throughput potential. The applicability of the assay for progesterone profiling during the menstrual cycle is demonstrated.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/0004563218780904","type":"journal-article","created":{"date-parts":[[2018,5,23]],"date-time":"2018-05-23T23:48:29Z","timestamp":1527119309000},"page":"64-71","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":11,"title":["A novel high-throughput assay for the measurement of salivary progesterone by liquid chromatography tandem mass spectrometry"],"prefix":"10.1177","volume":"56","author":[{"given":"Lina","family":"Schiffer","sequence":"first","affiliation":[{"name":"Institute of Metabolism and Systems Research, University of Birmingham, Birmingham, UK"},{"name":"Centre for Endocrinology, Diabetes and Metabolism, Birmingham Health Partners, Birmingham, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joanne 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