{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T03:54:47Z","timestamp":1793159687930,"version":"4.5.0"},"reference-count":21,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2020,10,6]],"date-time":"2020-10-06T00:00:00Z","timestamp":1601942400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Ann Clin Biochem"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>To date, the difference in microRNA expression profiles in tears of dry eye patients and healthy people has not been reported. In current study, we evaluated the significance of microRNAs and transforming growth factor beta2 (TGF-\u03b22) in distinguishing dry eye.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A total of 138 patients with dry eye from October 2017 to October 2018 were selected. During the same period, 138 healthy persons were collected. All patients were followed up for 12\u2009months through outpatient, telephone or medical records and the time of corneal injury was recorded.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Compared with healthy people, TGF-\u03b22 concentrations in dry eye patients were significantly decreased ( P\u2009&lt; \u20090.05). Array analysis, predictive software and dual-luciferase reporter assays showed that miR-450b-5p, miR-1283 and miR-3671 can target TGF-\u03b22 expression. Tear miR-450b-5p, miR-1283 and miR-3671 concentrations were significantly higher in dry eye patients than healthy people. A logistic regression model combining miR-450b-5p, miR-1283, miR-3671 and TGF-\u03b22 was performed. This model presented a high discriminating value (AUC: 0.907, 0.876\u20130.939, P\u2009&lt; \u20090.001) than any single indicator, and the sensitivity and specificity were 77.7% and 92.7%, respectively. Compared with the low miR-450b-5p, low miR-1283, low miR-3671 and high TGF-\u03b22 groups, the high miR-450b-5p, high miR-1283, high miR-3671 and low TGF-\u03b22 groups had a significantly higher probability of corneal injury (TGF-\u03b22: \u03c7\n                      <jats:sup>2<\/jats:sup>\n                      \u2009=\u20095.762, P\u2009=\u20090.016; miR-450b-5p: \u03c7\n                      <jats:sup>2<\/jats:sup>\n                      \u2009=\u200913.267, P\u2009&lt; \u20090.001; miR-1283: \u03c7\n                      <jats:sup>2<\/jats:sup>\n                      \u2009=\u200919.431, P\u2009&lt; \u20090.001; miR-3671: \u03c7\n                      <jats:sup>2<\/jats:sup>\n                      \u2009=\u20098.131, P\u2009=\u20090.004).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Current model combining tear miR-450b-5p, miR-1283, miR-3671 and TGF-\u03b22 had important values in the identification of dry eye and was of great value in evaluating the risk of corneal injury.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/0004563220961746","type":"journal-article","created":{"date-parts":[[2020,9,16]],"date-time":"2020-09-16T14:47:27Z","timestamp":1600267647000},"page":"420-428","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":22,"title":["Discovery of microRNA expression profiles involved in regulating TGF-\n                    <b>\u03b2<\/b>\n                    2 expression in the tears of dry eye patients"],"prefix":"10.1177","volume":"57","author":[{"given":"Qing","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Heji Hospital Affiliated to Changzhi Medical College, Changzhi, P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangrong","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Heji Hospital Affiliated to Changzhi Medical College, Changzhi, P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huilin","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Heji Hospital Affiliated to Changzhi Medical College, Changzhi, P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7009-2359","authenticated-orcid":false,"given":"Shaofeng","family":"Hao","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Heji Hospital Affiliated to Changzhi Medical College, Changzhi, P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2020,10,6]]},"reference":[{"key":"bibr1-0004563220961746","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtos.2018.07.005"},{"key":"bibr2-0004563220961746","doi-asserted-by":"publisher","DOI":"10.5603\/IMH.2020.0015"},{"key":"bibr3-0004563220961746","doi-asserted-by":"publisher","DOI":"10.1016\/j.abd.2018.07.002"},{"key":"bibr4-0004563220961746","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.19-27062"},{"key":"bibr5-0004563220961746","doi-asserted-by":"publisher","DOI":"10.3341\/kjo.2016.30.3.163"},{"key":"bibr6-0004563220961746","doi-asserted-by":"publisher","DOI":"10.1097\/ICO.0000000000002148"},{"key":"bibr7-0004563220961746","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtos.2017.05.007"},{"key":"bibr8-0004563220961746","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0174585"},{"key":"bibr9-0004563220961746","doi-asserted-by":"publisher","DOI":"10.1186\/s13075-016-0987-0"},{"key":"bibr10-0004563220961746","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2018.02.002"},{"key":"bibr11-0004563220961746","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2008.112797"},{"key":"bibr12-0004563220961746","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18081764"},{"key":"bibr13-0004563220961746","first-page":"113","volume":"112","author":"Bartoloni E","year":"2018","journal-title":"Clin Exp Rheumatol"},{"key":"bibr14-0004563220961746","doi-asserted-by":"publisher","DOI":"10.1097\/ACI.0000000000000471"},{"key":"bibr15-0004563220961746","doi-asserted-by":"publisher","DOI":"10.18240\/ijo.2020.03.18"},{"key":"bibr16-0004563220961746","doi-asserted-by":"publisher","DOI":"10.1186\/s12886-020-01395-z"},{"key":"bibr17-0004563220961746","doi-asserted-by":"publisher","DOI":"10.1111\/dgd.12630"},{"key":"bibr18-0004563220961746","doi-asserted-by":"publisher","DOI":"10.2147\/DDDT.S168897"},{"key":"bibr19-0004563220961746","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2019.08.004"},{"key":"bibr20-0004563220961746","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2010.09.043"},{"key":"bibr21-0004563220961746","doi-asserted-by":"publisher","DOI":"10.1097\/ICO.0b013e3181cf98ff"}],"container-title":["Annals of Clinical Biochemistry: International Journal of Laboratory Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0004563220961746","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/0004563220961746","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0004563220961746","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T12:24:13Z","timestamp":1777379053000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/0004563220961746"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10,6]]},"references-count":21,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2020,11]]}},"alternative-id":["10.1177\/0004563220961746"],"URL":"https:\/\/doi.org\/10.1177\/0004563220961746","relation":{},"ISSN":["0004-5632","1758-1001"],"issn-type":[{"value":"0004-5632","type":"print"},{"value":"1758-1001","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,10,6]]}}}