{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T02:01:39Z","timestamp":1787018499150,"version":"build-2736575974"},"reference-count":18,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2022,1,6]],"date-time":"2022-01-06T00:00:00Z","timestamp":1641427200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Ann Clin Biochem"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>In samples from patients administered rasburicase, ex vivo uricolysis leads to spuriously low uric acid results. The manufacturer\u2019s recommendation of storing the sample in ice-water until analysis, however, does not fully arrest uricolysis. Since uricase activity is affected by pH and metal chelators, we assessed uricolysis inhibition in sodium fluoride-ethylenediaminetetraacetic acid (EDTA)-citrate sample tube (FC Mix tube, Greiner) used primarily for plasma glucose.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>A serum pool was spiked with rasburicase and uric acid measured at 15, 45, 90, 150, 240 and 1080\u00a0min in a lithium heparin tube in ice-water, plain tube at room temperature (RT), EDTA tube at RT, FC Mix tube in ice-water, FC Mix tube at RT and FC Mix tube at RT prepared by dissolving FC Mix in serum.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The rate of urate decay was lowest in the FC Mix tube independent of temperature, then lithium heparin tube in ice-water, then EDTA tube at RT and highest in the plain tube at RT. Uric acid concentrations in the prepared FC Mix tube at RT and heparin tube in ice-water were, respectively, 98.2% and 93.8% of control values at 90\u00a0min, 97.1% and 89.3% of control values at 4\u00a0h, and remained higher in the prepared FC Mix tube at all time points.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>NaF-EDTA-citrate mixture largely arrested rasburicase mediated ex vivo uricolysis without the need for sample cooling. We propose that sample tubes containing NaF-EDTA-citrate be used for the measurement of uric acid in patients administered rasburicase.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/00045632211066275","type":"journal-article","created":{"date-parts":[[2022,1,6]],"date-time":"2022-01-06T05:58:43Z","timestamp":1641448723000},"page":"178-182","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Acid freezes uricolysis in addition to glycolysis: Utility of fluoride-EDTA-citrate tube in the measurement of uric acid for patients administered rasburicase"],"prefix":"10.1177","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1306-8832","authenticated-orcid":false,"given":"Tejas","family":"Kalaria","sequence":"first","affiliation":[{"name":"Clinical Biochemistry, New Cross Hospital, Black Country Pathology Services, The Royal Wolverhampton NHS Trust, Wolverhampton, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan","family":"Fenn","sequence":"additional","affiliation":[{"name":"Clinical Biochemistry, New Cross Hospital, Black Country Pathology Services, The Royal Wolverhampton NHS Trust, Wolverhampton, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Whitmill","sequence":"additional","affiliation":[{"name":"Clinical Haematology, New Cross Hospital, Black Country Pathology Services, The Royal Wolverhampton NHS Trust, Wolverhampton, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clare","family":"Ford","sequence":"additional","affiliation":[{"name":"Clinical Biochemistry, New Cross Hospital, Black Country Pathology Services, The Royal Wolverhampton NHS Trust, Wolverhampton, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7483-9253","authenticated-orcid":false,"given":"Rousseau","family":"Gama","sequence":"additional","affiliation":[{"name":"Clinical Biochemistry, New Cross Hospital, Black Country Pathology Services, The Royal Wolverhampton NHS Trust, Wolverhampton, UK"},{"name":"School of Medicine and Clinical Practice, University of Wolverhampton, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2022,1,6]]},"reference":[{"key":"bibr1-00045632211066275","unstructured":"RASBURICASE- British National Formulary (online). https:\/\/bnf.nice.org.uk\/drug\/rasburicase.html#indicationsAndDoses (accessed 22 August 2021)."},{"key":"bibr2-00045632211066275","volume-title":"Electronic Medicines Compendium 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