{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T02:44:09Z","timestamp":1792205049928,"version":"4.4.0"},"reference-count":25,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2023,3,24]],"date-time":"2023-03-24T00:00:00Z","timestamp":1679616000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Ann Clin Biochem"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The use of supine reference intervals instead of the corresponding seated reference intervals for seated plasma-free metanephrines (MNs) in pheochromocytoma\/paraganglioma (PPGL) screening has been controversial in recent years. Each clinical laboratory should choose the optimal sampling posture and diagnostic strategy according to local conditions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The reference population consisted of 736 cases aged 14\u201392\u00a0years old and the validation population consisted of 1068 patients aged 8\u201387\u00a0years old. Seated MNs were measured by liquid chromatography-tandem mass spectrometry (LC-MS\/MS), and the reference intervals and diagnostic cut-off values were established and the diagnostic performance compared with reference intervals established in a supine position.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>There was no correlation between seated plasma MNs and age ( p &gt; 0.05) and there were differences in MNs among the various disease groups ( p &lt; 0.05). MNs were different in gender ( p &lt; 0.0001). The upper reference limit (URL) established in this study had the same sensitivity (100%) and better specificity (94.6% vs 83.5%) compared with the published age-adjusted supine reference intervals. The proportion of suspected patients with MNs within the URL-2\u00d7URL range was lower using seated reference intervals compared to supine intervals (5.3% vs 15.7%).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Using the corresponding seated reference intervals for seated plasma MNs can reduce the unnecessary re-examinations of suspected patients with slightly elevated MNs. The cut-off value established by seated plasma MNs has good diagnostic performance in PPGL. Use of seated sampling is an acceptable practice and is more convenient and economical than supine sampling.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/00045632231154731","type":"journal-article","created":{"date-parts":[[2023,1,18]],"date-time":"2023-01-18T22:39:03Z","timestamp":1674081543000},"page":"160-168","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["Establishment of reference intervals for plasma metanephrines in seated position measured by LC-MS\/MS and assessment of diagnostic performance in pheochromocytoma\/paraganglioma"],"prefix":"10.1177","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8821-4844","authenticated-orcid":false,"given":"Yuxin","family":"Lin","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory, Fujian Medical University Union Hospital, Fuzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meihua","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Fujian Medical University Union Hospital, Fuzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingping","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Fujian Medical University Union Hospital, Fuzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2023,3,24]]},"reference":[{"key":"bibr1-00045632231154731","volume-title":"Clinical and experimental pheochromocytoma","author":"Manger WM","year":"1996","edition":"2"},{"key":"bibr2-00045632231154731","doi-asserted-by":"publisher","DOI":"10.1007\/s11906-018-0804-z"},{"key":"bibr3-00045632231154731","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2003.024703"},{"key":"bibr4-00045632231154731","volume-title":"Test Definition: CATP Catecholamine Fract, Free P","author":"Mayo Clinic Laboratories","year":"2020"},{"key":"bibr5-00045632231154731","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2014-1498"},{"key":"bibr6-00045632231154731","first-page":"737","volume":"36","author":"Chinese Society of Endocrinology Adrenal Group","year":"2020","journal-title":"Chin J Endocrinol Metlab"},{"key":"bibr7-00045632231154731","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.11.1.3"},{"key":"bibr8-00045632231154731","doi-asserted-by":"publisher","DOI":"10.1530\/EJE-19-0176"},{"key":"bibr9-00045632231154731","doi-asserted-by":"publisher","DOI":"10.1111\/cen.12327"},{"key":"bibr10-00045632231154731","doi-asserted-by":"publisher","DOI":"10.1177\/0004563216646395"},{"key":"bibr11-00045632231154731","doi-asserted-by":"publisher","DOI":"10.1111\/cen.12602"},{"key":"bibr12-00045632231154731","doi-asserted-by":"publisher","DOI":"10.1111\/cen.12587"},{"key":"bibr13-00045632231154731","doi-asserted-by":"publisher","DOI":"10.1111\/cen.12624"},{"key":"bibr14-00045632231154731","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2006.076489"},{"key":"bibr15-00045632231154731","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2010.01.012"},{"key":"bibr16-00045632231154731","doi-asserted-by":"publisher","DOI":"10.1507\/endocrj.EJ14-0384"},{"key":"bibr17-00045632231154731","doi-asserted-by":"publisher","DOI":"10.1177\/0004563216650463"},{"key":"bibr18-00045632231154731","doi-asserted-by":"publisher","DOI":"10.1258\/acb.2012.012066"},{"key":"bibr19-00045632231154731","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2018.12.019"},{"key":"bibr20-00045632231154731","doi-asserted-by":"publisher","DOI":"10.3803\/EnM.2017.32.2.152"},{"key":"bibr21-00045632231154731","first-page":"489","volume":"30","author":"Su TW","year":"2010","journal-title":"J Shanghai Jiaotong Univ (Med Sci)"},{"key":"bibr22-00045632231154731","first-page":"1982","volume":"11","author":"Huang F","year":"2017","journal-title":"Chin J Exp Surg"},{"key":"bibr23-00045632231154731","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2019.03.1620"},{"key":"bibr24-00045632231154731","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)67139-5"},{"key":"bibr25-00045632231154731","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2018.291369"}],"container-title":["Annals of Clinical Biochemistry: International Journal of Laboratory Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/00045632231154731","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/00045632231154731","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/00045632231154731","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T12:25:04Z","timestamp":1777379104000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/00045632231154731"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,24]]},"references-count":25,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2023,5]]}},"alternative-id":["10.1177\/00045632231154731"],"URL":"https:\/\/doi.org\/10.1177\/00045632231154731","relation":{},"ISSN":["0004-5632","1758-1001"],"issn-type":[{"value":"0004-5632","type":"print"},{"value":"1758-1001","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,24]]}}}