{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T06:41:03Z","timestamp":1777444863705,"version":"3.51.4"},"reference-count":30,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2023,3,3]],"date-time":"2023-03-03T00:00:00Z","timestamp":1677801600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Vestre Viken Hospital Trust"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Ann Clin Biochem"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The iron-regulatory hormone hepcidin is a promising biomarker to differentiate anaemia of inflammation from iron deficiency. Plasma hepcidin concentrations increase substantially during inflammation, and the amount of smaller, non-biologically active isoforms of hepcidin increase in inflammatory conditions. These smaller isoforms are measured in some, but not all analytical methods. Thus, we evaluated the comparability of two analytical methods with different isoform selectivity during and after acute-phase pneumonia as a highly inflammatory model disease.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Blood samples from a cohort of 267 hospitalized community-acquired pneumonia patients collected at admission and a 6-week follow-up were analysed. Hepcidin was measured in plasma by an immunoassay, which recognizes all hepcidin isoforms, and a liquid chromatography tandem mass spectrometry (LC-MS\/MS), which selectively measures the bioactive hepcidin-25. Additionally, a subset of serum samples was analysed by LC-MS\/MS.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Hepcidin measurements by immunoassay were higher compared with LC-MS\/MS. The relative mean difference of hepcidin plasma concentrations between the two analytical methods was larger in admission samples than in follow-up samples (admission samples &lt;200\u00a0ng\/mL: 37%, admission samples &gt;200\u00a0ng\/mL: 78%, follow-up samples &gt;10\u00a0ng\/mL: 22%). During acute-phase pneumonia, serum concentrations were on average 22% lower than plasma concentrations when measured by LC-MS\/MS.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Immunoassay measured higher hepcidin concentrations compared with LC-MS\/MS, with more pronounced differences in high-concentration samples during acute-phase pneumonia. These findings should be considered in local method validations and in future harmonization and standardization optimization of hepcidin measurements.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/00045632231159529","type":"journal-article","created":{"date-parts":[[2023,2,9]],"date-time":"2023-02-09T23:21:25Z","timestamp":1675984885000},"page":"298-305","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Hepcidin analysis in pneumonia: Comparison of immunoassay and LC-MS\/MS"],"prefix":"10.1177","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0853-5769","authenticated-orcid":false,"given":"Kjersti","family":"Oppen","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Drammen Hospital, Vestre Viken Hospital Trust, Norway"},{"name":"Research Institute of Internal Medicine, Oslo University Hospital Rikshospitalet, Norway"},{"name":"Institute of Clinical Medicine, University of Oslo, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2691-6052","authenticated-orcid":false,"given":"Cato","family":"Brede","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, Stavanger University Hospital, Norway"},{"name":"Department of Chemistry, Bioscience and Environmental Engineering, University of Stavanger, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00d8yvind","family":"Skadberg","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, Stavanger University Hospital, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Trude","family":"Steinsvik","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Drammen Hospital, Vestre Viken Hospital Trust, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1618-5022","authenticated-orcid":false,"given":"Jan Cato","family":"Holter","sequence":"additional","affiliation":[{"name":"Institute of Clinical Medicine, University of Oslo, Norway"},{"name":"Department of Microbiology, Oslo University Hospital, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annika E","family":"Michelsen","sequence":"additional","affiliation":[{"name":"Research Institute of Internal Medicine, Oslo University Hospital Rikshospitalet, Norway"},{"name":"Institute of Clinical Medicine, University of Oslo, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lars","family":"Heggelund","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Drammen Hospital, Vestre Viken Hospital Trust, Norway"},{"name":"Department of Clinical Science, Bergen Integrated Diagnostic Stewardship Cluster, Faculty of 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Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2023,3,3]]},"reference":[{"key":"bibr1-00045632231159529","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.17252"},{"key":"bibr2-00045632231159529","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-12-639112"},{"key":"bibr3-00045632231159529","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0075518"},{"key":"bibr4-00045632231159529","doi-asserted-by":"publisher","DOI":"10.1007\/s12185-015-1885-y"},{"key":"bibr5-00045632231159529","doi-asserted-by":"publisher","DOI":"10.4155\/bio-2016-0286"},{"key":"bibr6-00045632231159529","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2010.149187"},{"key":"bibr7-00045632231159529","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2012.08.006"},{"key":"bibr8-00045632231159529","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2009.140053"},{"key":"bibr9-00045632231159529","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2021.10.032"},{"key":"bibr10-00045632231159529","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2009.010322"},{"key":"bibr11-00045632231159529","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.23289"},{"key":"bibr12-00045632231159529","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2016.256768"},{"key":"bibr13-00045632231159529","doi-asserted-by":"publisher","DOI":"10.1515\/cclm-2018-0783"},{"key":"bibr14-00045632231159529","doi-asserted-by":"publisher","DOI":"10.1515\/cclm-2020-0928"},{"key":"bibr15-00045632231159529","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2011.12.015"},{"key":"bibr16-00045632231159529","doi-asserted-by":"publisher","DOI":"10.1515\/cclm-2014-1093"},{"key":"bibr17-00045632231159529","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-015-0803-5"},{"key":"bibr18-00045632231159529","doi-asserted-by":"publisher","DOI":"10.1080\/23744235.2021.2022194"},{"key":"bibr19-00045632231159529","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofab082"},{"key":"bibr20-00045632231159529","unstructured":"Magnusson 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