{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T06:41:30Z","timestamp":1777444890769,"version":"3.51.4"},"reference-count":28,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T00:00:00Z","timestamp":1700524800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100005006","name":"Asan Institute for Life Sciences, Asan Medical Center","doi-asserted-by":"publisher","award":["2016-635"],"award-info":[{"award-number":["2016-635"]}],"id":[{"id":"10.13039\/501100005006","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Ann Clin Biochem"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Immune checkpoints are involved in mechanisms by which tumours escape from the host immune system. Our aim was to evaluate acute myeloid leukaemia (AML) patients to determine expression levels of checkpoint molecules according to diagnosis and treatments, and to identify optimal candidates for checkpoint blockade.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Bone marrow (BM) samples were obtained from 279 AML patients at different disease status and from 23 controls. Flow cytometric analyses of PD-1 and PD-L1\/PD-L2 expression were performed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Programmed death-1 (PD-1) expression levels on CD8\n                      <jats:sup>+<\/jats:sup>\n                      T-cells at AML diagnosis were increased compared to controls. PD-L1 and PD-L2 expression levels on leukaemic cells at diagnosis were significantly higher in secondary AML than in\n                      <jats:italic>de novo<\/jats:italic>\n                      AML. PD-1 levels on CD8\n                      <jats:sup>+<\/jats:sup>\n                      and CD4\n                      <jats:sup>+<\/jats:sup>\n                      T-cells after allo-SCT were significantly higher than those at diagnosis and after CTx. PD-1 expression on CD8\n                      <jats:sup>+<\/jats:sup>\n                      T-cells increased in the acute GVHD group than in the non-GVHD group. The overall survival of patients with high PD-1 expression on CD8\n                      <jats:sup>+<\/jats:sup>\n                      T-cells was significantly shorter than that of patients with low PD-1 expression.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      In conclusion, patients who underwent allo-SCT exhibited high PD-1 expression, suggesting that allo-SCT increases PD-1 expression on T-cells, and the patients with high PD-1 expression on CD8\n                      <jats:sup>+<\/jats:sup>\n                      T-cells after allo-SCT showed the poor prognosis. For these patients, PD-1 blockade could be an immunotherapeutic strategy.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/00045632231184716","type":"journal-article","created":{"date-parts":[[2023,6,14]],"date-time":"2023-06-14T11:28:06Z","timestamp":1686742086000},"page":"79-89","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":4,"title":["Increased PD-1 expression of bone marrow T-cells in acute myeloid leukaemia patients after stem cell transplantation, and its association with overall survival"],"prefix":"10.1177","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6836-499X","authenticated-orcid":false,"given":"Eunkyoung","family":"You","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Inje University College of Medicine, Busan Paik Hospital, Busan, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chan-Jeoung","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young-Uk","family":"Cho","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seongsoo","family":"Jang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min Young","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Kyung Hee University School of Medicine and Kyung Hee University Hospital at Gangdong, Seoul, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hery","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Ulsan College of Medicine, Asan Medical Center Children\u2019s Hospital, Seoul, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyung Nam","family":"Koh","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Ulsan College of Medicine, Asan Medical Center Children\u2019s Hospital, Seoul, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ho Joon","family":"Im","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Ulsan College of Medicine, Asan Medical Center Children\u2019s Hospital, Seoul, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eun-Ji","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Hematology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Je-Hwan","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Hematology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyoo-Hyung","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Hematology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2023,11,21]]},"reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2011.12.009"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1203486"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc3239"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm730"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.271.5256.1734"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0830997100"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-03-206672"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-10-310425"},{"key":"e_1_3_5_10_2","doi-asserted-by":"crossref","unstructured":"Naval D Sreyashi B Guillermo GM et al. 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