{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T01:58:10Z","timestamp":1789696690478,"version":"4.0.1"},"reference-count":25,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2023,8,31]],"date-time":"2023-08-31T00:00:00Z","timestamp":1693440000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Ann Clin Biochem"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>The prevalence of vitamin B12 deficiency is high in at-risk populations with sometimes irreversible consequences. Beside total B12 (TVB12), active B12 (AVB12) is a promising first-line marker. Only Abbott AVB12 assays were largely evaluated and generally demonstrated benefit in clinical practice. More recently developed Roche AVB12 still requires some investigations.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Our study aimed to evaluate the Roche Elecsys\u00ae AVB12 immunoassay performance versus Roche Elecsys\u00ae TVB12 competition assay.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Design<\/jats:title>\n                    <jats:p>and Methods: We included 175 patients at Rouen University Hospital who had a TVB12 value &lt;300 pmol\/L. We evaluated performance of AVB12 by comparing the results with TVB12 and MMA values in case of disagreement.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Positive correlation was found between the AVB12 and TVB12. We found a disagreement between TVB12 and AVB12 in 18.8% of cases. Among 33 cases of disagreement, 76% had normal AVB12 but low TVB12, whereas 24% had low AVB12 and normal TVB12. Thirty-one MMA determinations were performed: 71% showed agreement between MMA and AVB12, versus 29% between MMA and TVB12. TVB12 reported a sensitivity (Se) at 66.7%, specificity (Sp) at 20%, positive predictive value (PPV) at 16.7% and negative predictive value (NPV) at 71.4% for the prediction of MMA elevation. We determined an optimized cut-off value of 45.5 pmol\/L for AVB12, which reported a Se 66.7%, Sp 60%, PPV 30.7%, and NPV 88.9%.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our results provide preliminary evidence that Roche AVB12 may offer better discrimination than Roche TVB12 in the diagnosis of vitamin B12 deficiency. Further more detailed evaluation is warranted.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/00045632231194157","type":"journal-article","created":{"date-parts":[[2023,7,28]],"date-time":"2023-07-28T04:43:20Z","timestamp":1690519400000},"page":"55-62","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":4,"title":["Preliminary evaluation of the diagnostic performance of Roche Elecsys\u00ae active vitamin B12 versus total vitamin B12 for vitamin B12 deficiency screening"],"prefix":"10.1177","volume":"61","author":[{"given":"Jennifer","family":"Guillerme","sequence":"first","affiliation":[{"name":"Department of General Biochemistry, Rouen University Hospital, Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3125-6941","authenticated-orcid":false,"given":"Guillaume","family":"Feugray","sequence":"additional","affiliation":[{"name":"Department of General Biochemistry, Normandie Univ, UNIROUEN, INSERM U1096 EnVI, F-76000 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e9l\u00e8ne","family":"Girot","sequence":"additional","affiliation":[{"name":"Department of General Biochemistry, Rouen University Hospital, Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9ry","family":"Brunel","sequence":"additional","affiliation":[{"name":"Department of General Biochemistry, Rouen University Hospital, Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muriel Quillard","family":"Muraine","sequence":"additional","affiliation":[{"name":"Department of General Biochemistry, Rouen University Hospital, F-76000 Rouen, CIC-CRB, INSERM U1404, F-76000 Rouen and Normandie UNIV, UNIROUEN, INSERMU1073 ADEN"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2023,8,31]]},"reference":[{"key":"bibr1-00045632231194157","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2017.40"},{"key":"bibr2-00045632231194157","doi-asserted-by":"publisher","DOI":"10.1016\/j.mayocpiqo.2019.03.002"},{"key":"bibr3-00045632231194157","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2016.00027"},{"key":"bibr4-00045632231194157","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2010.158154"},{"key":"bibr5-00045632231194157","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2012.01.016"},{"issue":"22","key":"bibr6-00045632231194157","volume":"9120","author":"Sobczy\u0144ska-Malefora A","year":"2022","journal-title":"Clin 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